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Product Information
Immunogen
Synthetic glycine conjugated to protein carrier, bovine serum albumin (BSA)
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG
Format
Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity
1 mg
Reconstitution
For reconstitution add 500 µl of sterile water and 500 µl of glycerol.
Storage
Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles.
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Recommended dilution
1: 2000 - 1: 10 000 (ELISA), 1 : 1000-1 : 5 000 (ICC)
Reactivity
Confirmed reactivity
glycine
Predicted reactivity
The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
| Compounds | Cross-reactivity ratio (a) | |
| Glycine-G-PC* | 1 | |
| β−Alanine-G-PC | 1/100 | |
| GABA-G-PC | 1/500 | |
| Taurine-G-PC | 1/1 000 | |
| L.Aspartate-G-PC | 1/20 000 | |
| L.Glutamate-G-PC | 1/>20 000 | |
| Free Glycine | 1/>50 000 |
G- glutaraldehyde, (a) Glycine-G-PC concentration/unconjugated or conjugated amio acid concentration at half displacement
Glycine-BSA Kd around 10-8M, which is a very high affinity and specificity.
For example of immunocytochemistry protocol check here.total IgG was purified by ammonium sulfate precipitation.
Background
Background
Glycine (Gly) is a smallest of amino acids found in proteins.
Product citations
Selected references
Rubio & Juiz (2004). Differential distribution of synaptic endings containing glutamate, glycine, and GABA in the rat dorsal cochlear nucleus. J. Comp. Neurol. 477: 253-272.
Van Zundert et al. (2000). Effects of chronic ethanol treatment on gamma-aminobutyric acid(A) and glycine receptors in mouse glycinergic spinal neurons. J. Pharmacol. Exp. ther. 1:423-429.
Van Zundert et al. (2000). Effects of chronic ethanol treatment on gamma-aminobutyric acid(A) and glycine receptors in mouse glycinergic spinal neurons. J. Pharmacol. Exp. ther. 1:423-429.
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