GnRHa | gonadotropin-releasing hormone agonist
AS05 088 | Clonality: Polyclonal | Host: Hen | Reactivity: GnRHa
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Product Information
Immunogen
GnRHa (des-Gly10, D-Ala6, Pro9-NHEt)-LHRH pGlu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg Pro-NHEt C56H78N16O12,FW 1167
Host
Chicken
Clonality
Polyclonal
Purity
Total IgY
Format
Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity
200 µl
Storage
Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA)
Recommended dilution
1: : 5 000 (ELISA)
Reactivity
Confirmed reactivity
GnRHa
Predicted reactivity
GnRHa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells. After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent.
Background
Background
Gonadotropin-releasing hormone (GnRH) is considered to stimulate LH secretion from the pituitary. This antibody is raised against an GnRH analog (GnRHa).
Product citations
Selected references
Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297.
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