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GnRHa | gonadotropin-releasing hormone agonist (20 ĩl)

AS05 087-20  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: GnRHa

GnRHa | gonadotropin-releasing hormone agonist (20 ĩl) in the group Antibodies Other Species / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 087-20)



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

GnRHa (des-Gly10, D-Ala6, Pro9-NHEt)-LHRH pGlu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg Pro-NHEt C56H78N16O12, FW 1167

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 20 ĩl
Reconstitution For reconstitution add 20 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA)
Recommended dilution

1: : 80 000 (ELISA)

Reactivity

Confirmed reactivity GnRHa
Predicted reactivity

GnRHa

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells, After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent

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Background

Background

Gonadotropin-releasing hormone (GnRH) is considered to stimulate LH secretion from the pituitary. This antibody is raised against an GnRH analog (GnRHa).

Product citations

Selected references Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297.
immunogen:

GnRHa (des-Gly10, D-Ala6, Pro9-NHEt)-LHRH pGlu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg Pro-NHEt C56H78N16O12, FW 1167

Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 20 ĩl
Reconstitution: For reconstitution add 20 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: ELISA (ELISA)
recommended dilution:

1: : 80 000 (ELISA)

Confirmed reactivity: GnRHa
predicted reactivity:

GnRHa

not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information (application): In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells, After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent
background:

Gonadotropin-releasing hormone (GnRH) is considered to stimulate LH secretion from the pituitary. This antibody is raised against an GnRH analog (GnRHa).

All references: Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297.

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