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GnRHa | gonadotropin-releasing hormone agonist (200 ĩl)

AS05 087  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: GnRHa

GnRHa | gonadotropin-releasing hormone agonist (200 ĩl) in the group Antibodies Other Species / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 087)



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

GnRHa (des-Gly10, D-Ala6, Pro9-NHEt)-LHRH pGlu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg Pro-NHEt C56H78N16O12, FW 1167

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA)
Recommended dilution

1: : 80 000 (ELISA)

Reactivity

Confirmed reactivity GnRHa
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells, After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent

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Background

Background

Gonadotropin-releasing hormone (GnRH) is considered to stimulate LH secretion from the pituitary. This antibody is raised against an GnRH analog (GnRHa).

Product citations

Selected references Amezawa et al. (2018). Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of a crude gonadotropin-releasing hormone analogue. . Fish Sci (2018). https://doi.org/10.1007/s12562-018-1185-6.
Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297.
immunogen:

GnRHa (des-Gly10, D-Ala6, Pro9-NHEt)-LHRH pGlu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg Pro-NHEt C56H78N16O12, FW 1167

Reconstitution: For reconstitution add 200 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 200 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA)
recommended dilution:

1: : 80 000 (ELISA)

Confirmed reactivity: GnRHa
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information (application): In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells, After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent
background:

Gonadotropin-releasing hormone (GnRH) is considered to stimulate LH secretion from the pituitary. This antibody is raised against an GnRH analog (GnRHa).

All references: Amezawa et al. (2018). Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of a crude gonadotropin-releasing hormone analogue. . Fish Sci (2018). https://doi.org/10.1007/s12562-018-1185-6.
Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297.

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