Goat anti-Chicken IgY (H&L), Affinity purified, Unconjugated

AS99 003 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (egg yolk immunoglobulin)

Product Information

Immunogen

Purified IgY (whole protein) emulsified in Freud's adjuvant

Host Goat

Clonality Polyclonal

Purity Affinity purified in two steps: total IgG. (1), followed by purification on Immunogen (2), in PBS pH 7.4.

Format Liquid

Quantity 2,5 mg (1,2 mg/ml)

Storage Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA)

Recommended dilution 1 : 8 000 (ELISA)

Expected | apparent MW

180 kDa

Reactivity

Confirmed reactivity Chicken IgY (egg yolk immunoglobulin)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Anti-IgY antibodies (0,1 mg/ml) at a dilution 1: 8 000 were found to generate absorbance 1,0 at 650 nm after 10 minutes of incubation with DAKO® One-Step Substrate System at room temperature

Background

IgY is an immunoglobulin found in a bird egg yolk – therefore named IgY. This protein is accumulated in egg yolk to provide passive immunoprotection to a hatched chick. On a molecular level IgY differs slightly from mammalian immunoglobulin found in serum called IgG.

Product citations

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calculated \| apparent molecular mass [kDa]:	180 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Goat
immunogen:	Purified IgY (whole protein) emulsified in Freud's adjuvant
Purity:	Affinity purified in two steps: total IgG. (1), followed by purification on Immunogen (2), in PBS pH 7.4.
Quantity:	2,5 mg (1,2 mg/ml)
recommended dilution:	1 : 8 000 (ELISA)
storage:	Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA)
background:	IgY is an immunoglobulin found in a bird egg yolk – therefore named IgY. This protein is accumulated in egg yolk to provide passive immunoprotection to a hatched chick. On a molecular level IgY differs slightly from mammalian immunoglobulin found in serum called IgG.
additional information (application):	Anti-IgY antibodies (0,1 mg/ml) at a dilution 1: 8 000 were found to generate absorbance 1,0 at 650 nm after 10 minutes of incubation with DAKO® One-Step Substrate System at room temperature
Confirmed reactivity:	Chicken IgY (egg yolk immunoglobulin)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known