showForm Goat anti-Chicken IgY (H&L), ALP conjugated

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Goat anti-Chicken IgY (H&L), ALP conjugated - trial

AS09 606-trial | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&L)

Goat anti-Chicken IgY (H&L), ALP conjugated - trial in the group Secondary Antibodies / Anti-Chicken  / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS09 606-trial)

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Product Information

Immunogen

purified chicken IgY, whole molecule

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Liquid
Quantity 10 µl
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 10 000-100 000 (WB)

Reactivity

Confirmed reactivity Chicken IgY heavy and light chains (H&L)
Predicted reactivity Chicken IgY Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)

Antibody is affinity purified using solid phase Chicken IgG/Y.

AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresis

chicken IgY is also called chicken or hen IgG

Related products

Related products

AS09 603 | Goat anti-chicken IgY (H&L) HRP conjugated

AS09 610 | Goat anti-chicken IgY (H&L) biotin conjugated

Background

Background

Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.

Product citations

Selected references

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