showForm Goat anti-rat IgG (H&L), F(ab)'2 fragment, ALP (alkaline phosph

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Goat anti-Rat IgG (H&L), F(ab)'2 fragment, ALP conjugated, min. cross-reactivity to human, mouse IgG

AS10 1206   | Clonality: Polyclonal  |  Host:Goat   |  Reactivity:Rat IgG (H&L)

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, ALP conjugated, min. cross-reactivity to human, mouse IgG in the group Secondary Antibodies / Anti-Rat / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1206)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

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Additional information

Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

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Background

Background Goat anti-rat IgG (H&L), ALP (alkaline phosphatase) conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rat IgG in immunological assays. Antibodies are adsorbed against human,mouse IgG. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

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