GM-CSF | Granulocyte-macrophage colony-stimulating factor

IMS01-120-318 | Clonality: Polyclonal | Host: Hen | Reactivity: Human

Product Information

Immunogen

purified full length recombinant protein UniProt: P04141

Host Chicken

Clonality Polyclonal

Purity Purified, total IgY (chicken egg yolk immunoglobulin) in 0.9% NaCl, 0.02% sodium azide.

Format Liquid

Quantity 100 µl (10,7 mg/ml)

Storage Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Total, purified IgY

Recommended dilution 1 : 1000-1 : 5 000 (ELISA), 1 : 1000-1 : 5 000 (WB)

Expected | apparent MW

16 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Primates, Rat

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information The IgY fraction is isolated by a two-step PEG precipitation, procedure followed by ammonium sulphate precipitation

The antibody, in dilution 1: 1000, will detect 10 ng of GM-CSF in immunoblotting

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Product citations

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calculated \| apparent molecular mass [kDa]:	16 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Chicken
immunogen:	purified full length recombinant protein UniProt: P04141
Purity:	Purified, total IgY (chicken egg yolk immunoglobulin) in 0.9% NaCl, 0.02% sodium azide.
Quantity:	100 µl (10,7 mg/ml)
recommended dilution:	1 : 1000-1 : 5 000 (ELISA), 1 : 1000-1 : 5 000 (WB)
storage:	Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Total, purified IgY
additional information:	The IgY fraction is isolated by a two-step PEG precipitation, procedure followed by ammonium sulphate precipitation
additional information (application):	The antibody, in dilution 1: 1000, will detect 10 ng of GM-CSF in immunoblotting
background:	Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.
Confirmed reactivity:	Human
predicted reactivity:	Primates, Rat
not reactive in:	No confirmed exceptions from predicted reactivity are currently known