GST class-phi | Glutathione S tranferase

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P46422, TAIR: At4g02520

24 | 26 kDa

Application example

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-GST antibodies (AS09 479, 1:1000, 1h) and secondary anti-rabbit (1:1000, 1 h). Detection was done using chemiluminescence. All steps were performed in RT with agitation.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufacture by Operon Biotechnologies.

antibodies to plant endomembrane proteins

Plant protein extraction buffer

Secondary antibodies

Glutathione S-transferase PM24 (EC=2.5.1.18) is an enzyme which performs conjugation of reduced glutathione to a range of exogenous and endogenous hydrophobic compunds. Alternative name: 24 kDa auxin-binding protein, glutathione S-transferse PM24