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GST class-phi | Glutathione S tranferase

AS09 479 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, L. formosa, S. superba

GST class-phi | Glutathione S tranferase in the group Antibodies Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS09 479)
GST class-phi | Glutathione S tranferase



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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P46422, TAIR: At4g02520

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

24 | 26 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Liquidambar formosa, Schima superba
Predicted reactivity Arabidopsis lyRatha, Brassica juncea, Brassica rapa, Bruguiera gymnorhiza, Capsella rubella,Capsicum annuum, Capsicum chinense, Gossypium hirsutum, Hyoscyamus muticus, Nicotiana sylvestris, Noccaea caerulescens, Physcomitrium patens, Solanum commersonii, Solanum tuberosum, Vigna radiata var. radiata
Species of your interest not listed? Contact us
Not reactive in Amaranthus tuberculatus

Application examples

Application examples

Application example

 

 

western blot detection using anti-GST antibodies

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-GST antibodies (AS09 479, 1:1000, 1h) and secondary anti-rabbit (1:1000, 1 h). Detection was done using chemiluminescence. All steps were performed in RT with agitation.

Reactant: Liquidambar formosana (Chinese sweet gum)

Application: Western Blotting

Pudmed ID: 25025692

Journal: PLoS One

Figure Number: 6A

Published Date: 2014-07-16

First Author: Chen, J., Hu, W. J., et al.

Impact Factor: 2.942

Open Publication

Western blot analysis showing the expression of three protein spots.(A) Expression of rubulose-1,5-bisphoshate carboxylase large subunit (RuBisco LSU), ascorbate peroxidase (APX) and glutathione-S-transferase (GST) in L. formosana and S. superba seedlings after AR treatment. Relative expression level of RuBisco LSU (B), APX (C) and GST (D) were analyzed with the Quantity One software. ?-actin was used as the internal control. Means with different letters indicate significantly difference (p<0.05) with regard to AR treatments.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufacture by Operon Biotechnologies.

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Background

Background

Glutathione S-transferase PM24 (EC=2.5.1.18) is an enzyme which performs conjugation of reduced glutathione to a range of exogenous and endogenous hydrophobic compunds. Alternative name: 24 kDa auxin-binding protein, glutathione S-transferse PM24

Product citations

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