Name:
Phone:
E-mail:
Address:






GST-tag, ALP-conjugated (mouse monoclonal, clone 3E2)

AS18 4185   | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: GST-tagged proteins

GST-tag, ALP-conjugated (mouse monoclonal, clone 3E2) in the group Tag Antibodies / GST/MBP/Trx at Agrisera AB (Antibodies for research) (AS18 4185)



DATA SHEET IN PDF

Qty: 
473
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen GST tagged protein from Schistosoma japonicum.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.
Format Liquid
Quantity 100 ĩg
Storage Store at 2-8°C.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 800 (ELISA), 1: 1000 (WB)
Expected | apparent MW 26 kDa

Reactivity

Application examples

Application examples Application example



Western blot detection of GST protein using Mouse Anti-GST Monoclonal Antibody (1: 1000 for 2h at RT), Clone 3E2. Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (1), GST (2). Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Chemiluminescent detection, exposure 5 min in RT. 

Additional information

For in vitro research use only

Related products

Background

Background GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.

Product citations

background: GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
calculated | apparent molecular mass [kDa]: 26 kDa
Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen: GST tagged protein from Schistosoma japonicum.
Purity: Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.
Quantity: 100 ĩg
recommended dilution: 1 : 800 (ELISA), 1: 1000 (WB)
storage: Store at 2-8°C.
Sub class: IgG1
tested applications: ELISA (ELISA), Western blot (WB)
Picture (footer): Application example



Western blot detection of GST protein using Mouse Anti-GST Monoclonal Antibody (1: 1000 for 2h at RT), Clone 3E2. Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (1), GST (2). Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Chemiluminescent detection, exposure 5 min in RT. 

additional information (application): For in vitro research use only

Related products: GST-tag, ALP-conjugated (mouse monoclonal, clone 3E2)

AS18 4186   | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: GST-t...
473 €
AS18 4187   | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: GST-t...
473 €
AS18 4188   | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: GST-t...
473 €
AS18 4189   | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: GST-t...
473 €