GST-tag, ALP-conjugated (mouse monoclonal, clone 3E2)

AS18 4185 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tagged proteins

Product Information

Immunogen GST tagged protein from Schistosoma japonicum.

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.

Format Liquid

Quantity 100 µg

Storage Store at 2-8°C.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 800 (ELISA), 1: 1000 (WB)

Expected | apparent MW 26 kDa

Reactivity

Application examples

Application examples Application example

Western blot detection of GST protein using Mouse Anti-GST Monoclonal Antibody (1: 1000 for 2h at RT), Clone 3E2. Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (1), GST (2). Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Chemiluminescent detection, exposure 5 min in RT.

Additional information

For in vitro research use only

Background

Background GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.

Product citations

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background:	GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
calculated \| apparent molecular mass [kDa]:	26 kDa
Clonality:	Monoclonal
Format:	Liquid
Host:	Mouse
immunogen:	GST tagged protein from Schistosoma japonicum.
Purity:	Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.
Quantity:	100 µg
recommended dilution:	1 : 800 (ELISA), 1: 1000 (WB)
storage:	Store at 2-8°C.
Sub class:	IgG1
tested applications:	ELISA (ELISA), Western blot (WB)
Picture (footer):	Application example Western blot detection of GST protein using Mouse Anti-GST Monoclonal Antibody (1: 1000 for 2h at RT), Clone 3E2. Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (1), GST (2). Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Chemiluminescent detection, exposure 5 min in RT.
additional information (application):	For in vitro research use only