IKP

GST-tag, ALP-conjugated (mouse monoclonal, clone 3E2)

Name: GST-tag, ALP-conjugated (mouse monoclonal, clone 3E2)
Brand: 7
SKU: AS18 4185
Price: 473 EUR
Availability: InStock

Product no: AS18 4185

AS18 4185 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tagged proteins

473 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Sub class:	IgG1
Immunogen:	GST tagged protein from Schistosoma japonicum.
Host:	Mouse
Clonality:	Monoclonal
Purity:	Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.
Format:	Liquid
Quantity:	100 �g
Storage:	Store at 2-8�C.
Tested applications:	ELISA (ELISA), Western blot (WB)
Recommended dilution:	1 : 800 (ELISA), 1: 1000 (WB)
Expected \| apparent MW:	26 kDa

Application Examples

Application example

Western blot detection of GST protein using Mouse Anti-GST Monoclonal Antibody (1: 1000 for 2h at RT), Clone 3E2. Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (1), GST (2). Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Chemiluminescent detection, exposure 5 min in RT.

Additional Information
Additional information (application): For in vitro research use only
Background

Background:

GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.

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