IKP

GST-tag, ALP-conjugated (mouse monoclonal, clone 3E2)

Product no: AS18 4185

AS18 4185   | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: GST-tagged proteins

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  • Product Info
  • Sub class: IgG1
    Immunogen: GST tagged protein from Schistosoma japonicum.
    Host: Mouse
    Clonality: Monoclonal
    Purity: Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at 2-8C.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 800 (ELISA), 1: 1000 (WB)
    Expected | apparent MW: 26 kDa
  • Application Examples
  • Application example



    Western blot detection of GST protein using Mouse Anti-GST Monoclonal Antibody (1: 1000 for 2h at RT), Clone 3E2. Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (1), GST (2). Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Chemiluminescent detection, exposure 5 min in RT. 

  • Additional Information
  • Additional information (application): For in vitro research use only
  • Background
  • Background: GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
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