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GST-tag, Streptavidin-conjugated (mouse monoclonal, clone 3E2)

AS18 4189   | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: GST-tagged proteins

GST-tag, Streptavidin-conjugated (mouse monoclonal, clone 3E2) in the group Antibodies for Human/Animal  / Tag Antibodies / GST at Agrisera AB (Antibodies for research) (AS18 4189)

DATA SHEET IN PDF

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457
How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Glutathione-S-Transferase (GST) tagged protein from Schistosoma japonicum.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Protein G purified.
Format Liquid in PBS pH 7.4. Contains 50% glycerol and 0.09% Sodium Azide.
Quantity 100 µg
Reconstitution Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Storage

Store at -20°C.

Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 800 (ELISA), 1: 1000 (WB)
Expected | apparent MW 26 kDa

Reactivity

Confirmed reactivity GST-tagged proteins

Application examples

Application examples
Western blot using anti-GST antibodies

Samples:
Molecular Weight Ladder (MW) (1)
Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (2)
GST (3)
Protein load/well 1 µg. Following transfer, the membrane was blocked with 5 % non-fat milk in TBS-T. Primary antibody was incubated at 1: 1000 dilution for 2 h/RT followed by washes and incubation with goat anti-mouse IgG, HRP conjugated secondary antibodies at 1: 5000 for 1h/RT.  The blot was washed and developed using chemiluminescence detection reagent according to manufacture's recommendations. 


Additional information

Related products

Background

Background GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.

Product citations

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