GST-tag, Streptavidin-conjugated (mouse monoclonal, clone 3E2)

AS18 4189 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tagged proteins

Product Information

Immunogen Glutathione-S-Transferase (GST) tagged protein from Schistosoma japonicum.

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.

Format Liquid

Quantity 100 µg

Reconstitution Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Storage Store at 2-8°C.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 800 (ELISA), 1: 1000 (WB)

Expected | apparent MW 26 kDa

Reactivity

Confirmed reactivity GST-tagged proteins

Application examples

Application examples
Western blot using anti-GST antibodies

Samples:
Molecular Weight Ladder (MW) (1)
Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (2)
GST (3)
Protein load/well 1 µg. Following transfer, the membrane was blocked with 5 % non-fat milk in TBS-T. Primary antibody was incubated at 1: 1000 dilution for 2 h/RT followed by washes and incubation with goat anti-mouse IgG, HRP conjugated secondary antibodies at 1: 5000 for 1h/RT. The blot was washed and developed using chemiluminescence detection reagent according to manufacture's recommendations.

Additional information

Background

Background GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.

Product citations

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Confirmed reactivity:	GST-tagged proteins
Picture (footer):	Samples: Molecular Weight Ladder (MW) (1) Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (2) GST (3) Protein load/well 1 µg. Following transfer, the membrane was blocked with 5 % non-fat milk in TBS-T. Primary antibody was incubated at 1: 1000 dilution for 2 h/RT followed by washes and incubation with goat anti-mouse IgG, HRP conjugated secondary antibodies at 1: 5000 for 1h/RT. The blot was washed and developed using chemiluminescence detection reagent according to manufacture's recommendations.
background:	GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
calculated \| apparent molecular mass [kDa]:	26 kDa
Clonality:	Monoclonal
Format:	Liquid
Host:	Mouse
immunogen:	Glutathione-S-Transferase (GST) tagged protein from Schistosoma japonicum.
Purity:	Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.
Quantity:	100 µg
recommended dilution:	1 : 800 (ELISA), 1: 1000 (WB)
Reconstitution:	Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
storage:	Store at 2-8°C.
Sub class:	IgG1
tested applications:	ELISA (ELISA), Western blot (WB)