GST-tag, Streptavidin-conjugated (mouse monoclonal, clone 3E2)
AS18 4189 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tagged proteins
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Molecular Weight Ladder (MW) (1)
Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein (2)
Protein load/well 1 µg. Following transfer, the membrane was blocked with 5 % non-fat milk in TBS-T. Primary antibody was incubated at 1: 1000 dilution for 2 h/RT followed by washes and incubation with goat anti-mouse IgG, HRP conjugated secondary antibodies at 1: 5000 for 1h/RT. The blot was washed and developed using chemiluminescence detection reagent according to manufacture's recommendations.