GST-tag (mouse monoclonal, clone GST.B6)

AS15 2861 | Clonality: monoclonal | Host: Mouse | Reactivity:GST-tagged proteins

Product Information

Immunogen GST protein

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2a

Purity Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide

Format Liquid

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB), Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Recommended dilution 1 : 5000 (0.2 µg/µl) (WB)

Expected | apparent MW

26 kDa

Reactivity

Confirmed reactivity Native and denatured forms of purified GST or GST fusion proteins.

Predicted reactivity Native and denatured forms of purified GST or GST fusion proteins

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.

Antibody is purified on Protein A.

Related products

collection of antibodies to various tags

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.