GST-tag (mouse monoclonal, clone GST.B6)
AS15 2861 | Clonality: monoclonal | Host: Mouse | Reactivity:GST-tagged proteins
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Product Information
Immunogen
GST protein
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG2a
Purity
Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Format
Liquid
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB), Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Recommended dilution
1 : 5000 (0.2 µg/µl) (WB)
Expected | apparent MW
26 kDa
Reactivity
Confirmed reactivity
Native and denatured forms of purified GST or GST fusion proteins.
Predicted reactivity
Native and denatured forms of purified GST or GST fusion proteins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.
Antibody is purified on Protein A.
Background
Background
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Product citations
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