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Tag Antibody Set 

Helicobacter pylori

AS01 020 | Clonality: Polyclonal |  Host: Hen | Reactivity: Helicobater pylori

Helicobacter pylori in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS01 020)

PRODUCT INFORMATION IN PDF

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332 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

whole cells of Helicobacter pylori, standard laboratory strain (17-1 e.g. CCUG 17875) emulsified with an adjuvant.

Host Chicken
Clonality Polyclonal
Purity Total IgY
Format Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity 200 µl
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 10 000-1 : 20 000 (ELISA), 1 : 500 (IL), 1 : 5000-1 : 20 000 (WB)

Reactivity

Confirmed reactivity Helicobater pylori

Application examples

Application examples

Application example

immunochistochemistry of Helicobater pylori  using anti-chicken antibody

Panel 1: secondary antibody only

A. - Hoechst, B. - anti- chicken Alexa 568, dilution 1: 500, C. - Hoechst and anti-chicken Alexa 568

Panel 2: primary and secondary antibody detection

A. - Hoechst, B. - chicken anti-Helicobacter pylori at 1: 200 and anti-chicken Alexa 568, dilution 1: 500, C. - Hoechst, chicken anti-Helicobacter pylori at 1: 200 and anti-chicken Alexa 568

Protocol Immunofluorescence for Helicobacter pylori cells:

Harvest Helicobacter pylori from blood agar plates in 0.9% NaCl Put onto coverslips in 24-well plate and spin down at 1500g for 5min Fix with 4% Paraformaldehyde in PBS Wash with PBS 5 min triton X-100 0.2% in PBS Wash with PBS
Block with 4% foetal calf serum in PBS
Chicken anti-Helicobacter pylori primary antibody (Agrisera cat# AS01 020) diluted in 4% FSC in PBS 1 hour at room temp
Wash with PBS
Goat anti-Chicken Alexa fluor 568 (Invitrogen cat# A11041) in PBS 30 min at room temperature
Wash with PBS and water
3 min Hoechst 1ug/ml in water
Wash in water
Mount

Courtesy Dr.  Phebe Verbrugghe, QEII Medical Centre, Australia

Additional information

Additional information

IgY concentration is 23.5 mg/ml

Western blot has been done using whole extracts of Helicobacter pylori. Antibody recognised several low and high molecular weight H. pylori proteins. Some of them are from the surface of bacteria.

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Background

Background

Helicobacter pylori is nowadays recognised as one of the most common human pathogens, and has been recently defined as a class 1 carcinogen. It has an ability to colonise gastric mucosa, where it causes chronic gastritis and is a major cause for duodenal and sometimes gastric ulcers.

Product citations

Selected references Collins et al. (2016). The Helicobacter pylori CZB cytoplasmic chemoreceptor TlpD forms an autonomous polar chemotaxis signaling complex that mediates a tactic response to oxidative stress. J Bacteriol. 2016 Mar 21. pii: JB.00071-16.
Lertsethtakarn et al. (2015). Helicobacter pylori CheZHP and ChePep form a novel chemotaxis-regulatory complex distinct from the core chemotaxis signaling proteins and the flagellar motor. Mol Microbiol. 2015 Jun 10. doi: 10.1111/mmi.13086.

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