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HemH | Protoporphyrin ferrochelatase (cyanobacterial)

AS10 693  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:cyanobacteria

HemH | Protoporphyrin ferrochelatase (cyanobacterial) in the group Antibodies for Plant/Algal  / Photosynthesis  / Chlorophyll at Agrisera AB (Antibodies for research) (AS10 693)

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Product Information

Immunogen

KLH-conjugated peptide derived from cyanobacterial ferrochelatase protein sequence

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

46 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803
Predicted reactivity Cyanobacteria
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

 

western blot using anti-ferrochelatase antibodies (cyanobacterial)

Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon, Czech Republic

Additional information

Loads higher than 0.5 µg of chlorophyll per well are not recommended.

Related products

Related products

AS07 228 | Anti-HemH | protoporphyrin ferrochelatase (reacts with higher plants), rabbit antibodies

Algal protein extraction buffer

Secondary antibodies

Background

Background

HemH is ptotoporphyrin ferrochelatase of heme biosynthesis.

Product citations

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