HemH | Protoporphyrin ferrochelatase (cyanobacterial)
AS10 693 | Clonality: Polyclonal | Host: Rabbit | Reactivity:cyanobacteria
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KLH-conjugated peptide derived from cyanobacterial ferrochelatase protein sequence
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Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon, Czech Republic
HemH is ptotoporphyrin ferrochelatase of heme biosynthesis.