HemH | Protoporphyrin ferrochelatase (cyanobacterial)

AS10 693 | Clonality: Polyclonal | Host: Rabbit | Reactivity:cyanobacteria

Product Information

Immunogen

KLH-conjugated peptide derived from cyanobacterial ferrochelatase protein sequence

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW

46 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803

Predicted reactivity Cyanobacteria
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

western blot using anti-ferrochelatase antibodies (cyanobacterial)

Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon, Czech Republic