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Hirudin | Refludan

IMS01-134-330  |  Clonality: Polyclonal  |  Host: hen  |  Reactivity: Human

Hirudin | Refludan in the group Antibodies for Human/Animal  / Human Proteins / Immunology at Agrisera AB (Antibodies for research) (IMS01-134-330)

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Product Information

Immunogen

purified full length recombinant protein (Refludan®, Hoechst Marion Roussel Inc.)

Host Chicken
Clonality Polyclonal
Purity Total IgY fraction
Format Liquid in 0.9% NaCl, 0.1% sodium azide
Quantity 100 µl (18 mg/ml)
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Total, purified IgY
Recommended dilution 1 : 1000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

7 kDa

Reactivity

Confirmed reactivity Hirudin
Predicted reactivity

Hirudo medicinalis

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information The IgY fraction is isolated by a two-step PEG precipitation. procedure followed by ammonium sulphate precipitation.
Reactiv against purified hirudine; no reactivity against normal human plasma in ELISA.

Related products

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Secondary antibodies

Background

Background

Hirudin (P01050) is a highly effective and very specific anticoagulant which specifically inhibits formation of  fibrin and activation of factors V, VIII and XIII, by forming irreversible, noncovalent 1:1 complexes with alpha-thrombin. Hirudin is a 65 amino acid polypeptide, 7 kDa protein, naturally originating from the medical leech Hirudo medicinalis. It is a very potent thrombin inhibitor. Antibody binding to hirudin inhibits the interaction with alpha-thrombin.

Product citations

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