HTR12 | Histone H3-like centromeric protein

AS12 2226-100 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


product information
Background		Histone H3-like centromeric protein HTR12 replaces formal H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for the assembly of kinetochore proteins, mitotic progression and chromosome segregation. Localized at centromeres in both m itotic and meiotic cells. Synonymes: CENH3
Immunogen		KLH-conjugated synthetic peptide derived from N-terminal domain of Arabidopsis thaliana HTR12 AT1G01370 (Q8RVQ9)
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		100 µl
Reconstitution		For reconstitution add 100 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Store reconstituted antibodies at 4°C. Shelf life is 6 months from a date of receipt.
Tested applications		ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related products		AS12 2226-200 \| anti-HTR12 \| histone H3-like centromeric protein (200 µl) antibody Plant protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1 : 50-1 : 500 (IHC), 1 : 200-1 : 2000 (WB)
Expected \| apparent MW		17 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Crucihimalaya himalaica, Turritis glabra
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This product can be sold containing ProClin if requested
Selected references		to be added when available, antibody released in August 2012.

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