IKP

HliA | high light inducible protein

Product no: AS10 1603
AS10 1603 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803
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  • Product Info
  • Immunogen: Synthetic peptide (amino acids 1 – 17) specific for HliA protein from Synechocystis sp. PCC 6803, NP_440529.1
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Liquid
    Quantity: 50 l
    Storage: Store short-term 4C and long term at -20C. Repeated freezing and thawing is not recommended.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000-1 : 4000 (WB)
    Expected | apparent MW: 7,8 kDa
  • Reactivity
  • Confirmed reactivity: Synechocystis sp. PCC 6803
    Predicted reactivity: Synechocystis sp. PCC 6803
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-HliA antibodies

    25 µg of total protein extract from Synechocystis PCC6803 subjected to a high light stress, was extracted with TBS and denatured at 95ºC/10 min. andwas separated on a 12 % Tricine gel (containing 1%SDS and 10% APS)  and blotted to PVDF membrane (0.25 µm). The membrane was blocked with 10 % nonfat milk for 1h/RT and incubated with anti-HliA antibodies at 1: 3000 ON/4ºC, followed by washes and incubation with goat anti-rabbit HRP conjugated secondary antibodies and visiualized with chemiluminescent detection, following manufacture's recommendation. 

    Courtesy of Dr. Luna Viggiano de Alvarenga, Universität Rostock, Germany
  • Additional Information
  • Additional information: Antibody crossreacts with HliA and HliB, These two proteins can be separated by high-resolution gel electrophoresis,Pre-immune serum for this product is available upon request
  • Background
  • Background: HliA/B (SpcA/B) proteins are LHC-similar stress-induced proteins from cyanobacteria. This group of proteins is associated with damaged photosystem II and can serve as a temporary pigment reservoir while photosystem II components are being replaced.
  • Product Citations
  • Selected references: Aznar et al. (2020). Psb35 protein stabilizes the CP47 assembly module and associated high-light inducible proteins during the biogenesis of photosystem II in the cyanobacterium synechocystis sp. PCC6803. Plant Cell Physiol. 2020 Dec 1:pcaa148. doi: 10.1093/pcp/pcaa148. Epub ahead of print. PMID: 33258963.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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