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HliA | high light inducible protein

AS10 1603 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803
HliA | high light inducible protein in the group Antibodies for Plant/Algal  / Photosynthesis  / Proteases at Agrisera AB (Antibodies for research) (AS10 1603)

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Product Information

Immunogen Synthetic peptide (amino acids 1 – 17) specific for HliA protein from Synechocystis sp. PCC 6803, NP_440529.1
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 50 µl
Storage Store short-term 4°C. Long term -20°C. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000-1 : 4000 (WB)
Expected | apparent MW

7.8 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803
Predicted reactivity Synechocystis sp. PCC 6803
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-HliA antibodies

25 µg of total protein extract from Synechocystis PCC6803 subjected to a high light stress, was extracted with TBS and denatured at 95ºC/10 min. andwas separated on a 12 % Tricine gel (containing 1%SDS and 10% APS)  and blotted to PVDF membrane (0.25 µm). The membrane was blocked with 10 % nonfat milk for 1h/RT and incubated with anti-HliA antibodies at 1: 3000 ON/4ºC, followed by washes and incubation with goat anti-rabbit HRP conjugated secondary antibodies and visiualized with chemiluminescent detection, following manufacture's recommendation. 

Courtesy of Dr. Luna Viggiano de Alvarenga, Universität Rostock, Germany

Additional information

Additional information

Antibody crossreacts with HliA and HliB. These two proteins can be separated by high-resolution gel electrophoresis.

Pre-immune serum for this product is available upon request.

Related products

Background

Background HliA/B (SpcA/B) proteins are LHC-similar stress-induced proteins from cyanobacteria. This group of proteins is associated with damaged photosystem II and can serve as a temporary pigment reservoir while photosystem II components are being replaced.

Product citations

Selected references Aznar et al. (2020). Psb35 protein stabilizes the CP47 assembly module and associated high-light inducible proteins during the biogenesis of photosystem II in the cyanobacterium synechocystis sp. PCC6803. Plant Cell Physiol. 2020 Dec 1:pcaa148. doi: 10.1093/pcp/pcaa148. Epub ahead of print. PMID: 33258963.

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