HRP | Horseradish peroxidase (affinity purified)

AS09 549 | Clonality: Polyclonal | Host:Rabbit | Reactivity: Armoracia rusticana

Product Information

Immunogen

native peroxidase isolated and purified from horseradish

Host Rabbit

Clonality Polyclonal

Purity Affinity purified IgG

Format Lyophilized in phosphate buffer saline

Quantity 1 mg

Reconstitution For reconstitution add 0.5 ml of sterile destilled water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Recommended dilution 1 : 1000-1 : 10 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

40 kDa

Reactivity

Confirmed reactivity Armoracia rusticana

Predicted reactivity

Armoracia rusticana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Related products

AS16 3951 | Anti-HRP | Horseradish peroxidase, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.

Product citations

Selected references Rup et al. (2017). Immunogenicity of glycans on biotherapeutic drugs produced in plant expression systems-The taliglucerase alfa story. PLoS One. 2017 Oct 31;12(10):e0186211. doi: 10.1371/journal.pone.0186211.