HRP | Horseradish peroxidase (affinity purified)
AS09 549 | Clonality: Polyclonal | Host:Rabbit | Reactivity: Armoracia rusticana
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Product Information
Immunogen
native peroxidase isolated and purified from horseradish
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified IgG
Format
Lyophilized in phosphate buffer saline
Quantity
1 mg
Reconstitution
For reconstitution add 0.5 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Recommended dilution
1 : 1000-1 : 10 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
40 kDa
Reactivity
Confirmed reactivity
Armoracia rusticana
Predicted reactivity
Armoracia rusticana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
Background
Background
Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.
Product citations
Selected references
Rup et al. (2017). Immunogenicity of glycans on biotherapeutic drugs produced in plant expression systems-The taliglucerase alfa story. PLoS One. 2017 Oct 31;12(10):e0186211. doi: 10.1371/journal.pone.0186211.
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