showForm Anti-HRP | horseradish peroxidase (affinity purified) antibodies

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HRP | Horseradish peroxidase (affinity purified)

AS09 549 | Clonality: Polyclonal | Host:Rabbit | Reactivity: Armoracia rusticana

HRP | Horseradish peroxidase (affinity purified) in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS09 549)

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How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

native peroxidase isolated and purified from horseradish

Host Rabbit
Clonality Polyclonal
Purity Affinity purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 1 mg
Reconstitution For reconstitution add 0.5 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Recommended dilution 1 : 1000-1 : 10 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

40 kDa

Reactivity

Confirmed reactivity Armoracia rusticana
Predicted reactivity

Armoracia rusticana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Related products

Related products

AS09 548| anti-HRP horseradish

Plant protein extraction buffer

Secondary antibodies

Background

Background

Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.

Product citations

Selected references Rup et al. (2017). Immunogenicity of glycans on biotherapeutic drugs produced in plant expression systems-The taliglucerase alfa story. PLoS One. 2017 Oct 31;12(10):e0186211. doi: 10.1371/journal.pone.0186211.