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A1M | Human alpha-1-microglobulin

AS13 2670 | Clonality: Polyclonal |  Host: Hen | Reactivity: Human

A1M | Human alpha-1-microglobulin in the group Antibodies Human Cell Biology / Other proteins at Agrisera AB (Antibodies for research) (AS13 2670)



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387 €
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Product Information

Immunogen Full-length human alpha-1-microglobulin (A1M), purified from human urine,
Host Chicken
Clonality Polyclonal
Purity Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Format Liquid
Quantity 100 ĩg
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Radioimmunoassay (RIA), Western blot (WB)
Recommended dilution 1 : 5000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

27 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Human, Rabbit, Cat, Rat, Macaca fascicularis, Pan troglodytes, Gorilla, Ailuropoda melanoleuca
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information

IgY concentration is 19,8 mg/ml

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Background

Background

Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.

Product citations

Selected references Rutardottir et al. (2016). The cysteine 34 residue of A1M/α1-microglobulin is essential for protection of irradiated cell cultures and reduction of carbonyl groups. Free Radic Res. 2013 Jul;47(6-7):541-50. doi: 10.3109/10715762.2013.801555. Epub 2013 May 29.
Olsson MG et al. (2013). The radical-binding lipocalin A1M binds to a Complex I subunit and protects mitochondrial structure and function. Antioxid Redox Signal. 2013 Jun 1;18(16):2017-28.
calculated | apparent molecular mass [kDa]:

27 kDa
Clonality: Polyclonal
Format: Liquid
Host: Chicken
immunogen: Full-length human alpha-1-microglobulin (A1M), purified from human urine,
Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Quantity: 100 ĩg
recommended dilution: 1 : 5000 (ELISA), 1 : 2000 (WB)
storage: Store lyophilized/reconstituted at -20°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Radioimmunoassay (RIA), Western blot (WB)
All references: Rutardottir et al. (2016). The cysteine 34 residue of A1M/α1-microglobulin is essential for protection of irradiated cell cultures and reduction of carbonyl groups. Free Radic Res. 2013 Jul;47(6-7):541-50. doi: 10.3109/10715762.2013.801555. Epub 2013 May 29.
Olsson MG et al. (2013). The radical-binding lipocalin A1M binds to a Complex I subunit and protects mitochondrial structure and function. Antioxid Redox Signal. 2013 Jun 1;18(16):2017-28.
Confirmed reactivity: Human
predicted reactivity: Human, Rabbit, Cat, Rat, Macaca fascicularis, Pan troglodytes, Gorilla, Ailuropoda melanoleuca
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background:

Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.
additional information:

IgY concentration is 19,8 mg/ml

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