IBA | Indole-3-butyric acid (rabbit antibody)
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The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract.
Antibody titer in ELISA is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance. Plates can be coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-rabbit IgG can be used as detecting antibody.
Cross Reactivity %
Indole-3-aceteic acid < 0.1 %
Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.
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