IBA | Indole-3-butyric acid (rabbit antibody)

AS13 2744 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Indole-3-butyric acid


product information
Background		Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.
Immunogen		BSA-conjugated Indole-3-butyric acid, Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4(SIGMA), Solubility target: Methanol, ethanol.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Protein A purified total IgG
Format		Liquid
Quantity		1 ml (2.681 mg/ml)
Reconstitution
Storage		Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing.
Tested applications		ELISA (ELISA), Immunoaffinity chromatography (IAC)
Related products		AS15 3053 \| anti-IBA \| Indole-3-butyric acid , chicken antibodies AS09 445 \| anti-indole-3- acetic acid (C1') for immunolocalization, rabbit antibodies AS09 420 \| anti-indole 3 acetic acid (C1') (for ELISA), rabbit antibody AS09 421 \| anti-indole 3 acetic acid (N1), rabbit antibody AS11 1749 \| IAA \| auxin ELISA quantitation kit AS11 1749 \| IAA \| auxin ELISA quantitation kit AS12 1865 \| anti-Ethylene insensitive 2, rabbit antibody other Auxin antibodies collection of antibodies to plant hormones Secondary antibodies
Additional information		As antibodies to BSA carrier were not removed from this preparation please do not use BSA in your assay and use OVA-conjugated IBA for coating in ELISA.

application information
Recommended dilution		1 : 50 000 (indirect ELISA), 1 : 12 800 (direct ELISA)
Expected \| apparent MW
Confirmed reactivity		Indole-3-butyric acid (IBA)
Predicted reactivity
Not reactive in
Additional information		The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract. Antibody titer in ELISA is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance. Plates can be coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-rabbit IgG can be used as detecting antibody. Cross Reactivity % Indole-3-aceteic acid < 0.1 %
Selected references		to be added when available, antibody released in November 2013.

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