IBA | Indole-3-butyric acid (rabbit antibody)

AS13 2744 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Indole-3-butyric acid

Product Information

Immunogen BSA-conjugated Indole-3-butyric acid, Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4(SIGMA), Solubility target: Methanol, ethanol.

Host Rabbit

Clonality Polyclonal

Purity Protein A purified total IgG

Format Liquid

Quantity 2,6 mg (at 2,681 mg/ml)

Storage Store at 4°C up to one month or in aliquots at -20°C for long time storage, Avoid repeated freezing and thawing

Tested applications ELISA (ELISA), Immunoaffinity chromatography (IAC)

Recommended dilution 1 : 50 000 (indirect ELISA), 1 : 12 800 (direct ELISA)

Reactivity

Confirmed reactivity Indole-3-butyric acid (IBA)

Application examples

Additional information

Additional information As antibodies to BSA carrier were not removed from this preparation please do not use BSA in your assay and use OVA-conjugated IBA for coating in ELISA

The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract.
Antibody titer in ELISA is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance. Plates can be coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-rabbit IgG can be used as detecting antibody.

Cross Reactivity %
Indole-3-aceteic acid < 0.1 %

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other Auxin antibodies

collection of antibodies to plant hormones

Background

Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.

Product citations

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