IRT1 | Ion transporter-like protein
AS18 4173 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare
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recombinat protein of...expressed in E.coli
native, purified protein from
Affinity purified serum, in PBS pH 7.4
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
µg of total protein from extracted with write exact buffer components ..... and denatured with....at C or 70C for 2-5 min.... were separated on % SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:0 000 in for 1h at RT with agitation. The blot was washed as above and developed for min with EXACT NAME OF REAGENT AND SUPPLIER. Exposure time was seconds.
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