KARI | Ketol-acid reductoisomerase
AS15 2879 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, S. oleracea
|Data sheet||Product citations||Add review|
Species of your interest not listed? Contact us
15 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 (for sample 1) and 1: 2000 (for samples 2,3,4,5) for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 seconds.
Ketol-acid reductoisomerase is a catalytic enzyme in the biosynthetic pathway of amino acid biosynthesis. This protein catalyzes the reductive isomerization of 2-acetolactate to 2,3-dihy-droxyisovalerate.
Related products: KARI | Ketol-acid reductoisomerase
This product can b...