showForm KARI | Ketol-acid reductoisomerase

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KARI | Ketol-acid reductoisomerase

AS15 2879  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  A. thaliana, H. vulgare, N. tabacum, S. oleracea

KARI | Ketol-acid reductoisomerase               in the group Antibodies for Plant/Algal  / Developmental Biology / Senescence/Cell death at Agrisera AB (Antibodies for research) (AS15 2879)

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How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Full length, recombinant protein KARI protein from Spinacia oleracea, UniProt: Q01292,  TAIR:AT3G58610

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 3000-10 000 (WB)
Expected | apparent MW

63.75 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea,
Predicted reactivity Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-plant KARI antibodies
15 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 (for sample 1) and 1: 2000 (for samples 2,3,4,5) for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 seconds.

Additional information

Related products

Background

Background

Ketol-acid reductoisomerase is a catalytic enzyme in the biosynthetic pathway of amino acid biosynthesis. This protein catalyzes the reductive isomerization of 2-acetolactate to 2,3-dihy-droxyisovalerate.

Product citations

Selected references to be added when available, antibody released in May 2015