KARI | Ketol-acid reductoisomerase

Name: KARI | Ketol-acid reductoisomerase
Brand: IKP
SKU: AS15 2879
Price: 413 EUR
Availability: InStock

AS15 2879 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, S. oleracea

KARI | Ketol-acid reductoisomerase in the group Antibodies Plant/Algal / Developmental Biology / Senescence/Cell death at Agrisera AB (Antibodies for research) (AS15 2879)

Product Information

Immunogen

Full length, recombinant protein KARI protein from Spinacia oleracea, UniProt: Q01292, TAIR:AT3G58610

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000-10 000 (WB)

Expected | apparent MW 63,75 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea,

Predicted reactivity Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-plant KARI antibodies

15 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 (for sample 1) and 1: 2000 (for samples 2,3,4,5) for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 seconds.

Additional information

Background

Ketol-acid reductoisomerase is a catalytic enzyme in the biosynthetic pathway of amino acid biosynthesis. This protein catalyzes the reductive isomerization of 2-acetolactate to 2,3-dihy-droxyisovalerate.

Product citations

Picture (footer):	application example 15 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 (for sample 1) and 1: 2000 (for samples 2,3,4,5) for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 seconds.
Confirmed reactivity:	Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea,
predicted reactivity:	Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Ketol-acid reductoisomerase is a catalytic enzyme in the biosynthetic pathway of amino acid biosynthesis. This protein catalyzes the reductive isomerization of 2-acetolactate to 2,3-dihy-droxyisovalerate.
calculated \| apparent molecular mass [kDa]:	63,75 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Full length, recombinant protein KARI protein from Spinacia oleracea, UniProt: Q01292, TAIR:AT3G58610
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 3000-10 000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)