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LAC4 | Laccase

AS16 4073   | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Populus trichocarpa

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LAC4 | Laccase in the group Antibodies for Plant/Algal  / Cell Wall at Agrisera AB (Antibodies for research) (AS16 4073)

DATA SHEET IN PDF

How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide derived from 
recombinat protein of...expressed in E.coli
native, purified protein from http://www.uniprot.org/uniprot/B9IG56
Host Rabbit
Clonality Polyclonal
Purity Serum
Affinity purified serum, in PBS pH 7.4
Format

Lyophilized
Liquid

Quantity

 µg or µl

Reconstitution

For reconstitution add  µl, of sterile water.

Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution

1 : 1000 with ECL (WB)

Expected | apparent MW

 kDa

Reactivity

Confirmed reactivity Populus sp.
Predicted reactivity Betula platyphylla, Brassica oleracea, Eutrema salsugineum, Gossypium hirsutum, Helianthus annuus, Populus trichocarpa, Punica granatum, Vitis vinifera
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example





µg of total protein from    extracted with write exact buffer components ..... and denatured with....at C or 70C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:0 000 in  for 1h at RT with agitation. The blot was washed as above and developed for   min with   EXACT NAME OF REAGENT AND SUPPLIER. Exposure time was  seconds.

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Additional information

Related products

Related products AS

Background

Background Laccase is an enzyme involved in lignin degradation and detoxification of lignin-derived products. Alternative names: Benzenediol:oxygen oxidoreductase, Diphenol oxidase, Urishiol oxidase

Product citations

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