LAC4 | Laccase

Lyophilized
Liquid

 µg or µl

For reconstitution add  µl, of sterile water.

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

1 : 1000 with ECL (WB)

 kDa

No confirmed exceptions from predicted reactivity are currently known

µg of total protein from    extracted with write exact buffer components ..... and denatured with....at C or 70C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:0 000 in  for 1h at RT with agitation. The blot was washed as above and developed for   min with   EXACT NAME OF REAGENT AND SUPPLIER. Exposure time was  seconds.

Courtesy of