LOX2 | Lipoxygenase 2

AS08 340 | Clonality: Monoclonal | Host: Mouse | Reactivity: Triticum aestivum

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum LOX2 sequence

Host Mouse

Clonality Monoclonal

Purity Total IgG. Protein G purified purified from Cell culture supernatant.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Glycerol 1:1 can be added to improve the stability of this antibody.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 10 000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

90 kDa

Reactivity

Confirmed reactivity Triticum aestivum

Predicted reactivity Hordeum vulgare, Oryza sativa
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information LOX2 protein seems to be antigenically different compare to LOX isoforms isolated from leaves and flowers therefore this antibody being a monoclonal will not detect LOX2 from seeds, Brady et al (2014), Lipids: Structure and Function: The Biochemistry of Plants

Background

Lipoxygenases are a family of enzymes involved in oxidative stress response. They catalyse the oxygenation of polyunsaturated fatty acids to lipidhydroperoxides. LOX’s are known to play a role in plant growth and development.

Product citations

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background:	Lipoxygenases are a family of enzymes involved in oxidative stress response. They catalyse the oxygenation of polyunsaturated fatty acids to lipidhydroperoxides. LOX’s are known to play a role in plant growth and development.
additional information:	LOX2 protein seems to be antigenically different compare to LOX isoforms isolated from leaves and flowers therefore this antibody being a monoclonal will not detect LOX2 from seeds, Brady et al (2014), Lipids: Structure and Function: The Biochemistry of Plants
calculated \| apparent molecular mass [kDa]:	90 kDa
Clonality:	Monoclonal
Format:	Lyophilized
Host:	Mouse
immunogen:	KLH-conjugated synthetic peptide chosen from Triticum aestivum LOX2 sequence
Purity:	Total IgG. Protein G purified purified from Cell culture supernatant.
Quantity:	50 µg
recommended dilution:	1 : 10 000 (ELISA), 1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Glycerol 1:1 can be added to improve the stability of this antibody.
tested applications:	ELISA (ELISA), Western blot (WB)
Confirmed reactivity:	Triticum aestivum
predicted reactivity:	Hordeum vulgare, Oryza sativa Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known