LRIG1 | Lig-1 (rabbit antibodies)
AS18 4165 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human
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Product Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
143 kDa
Reactivity
Human, Hominidae,
no confirmed exceptions from predicted reactivity known in the moment
Application examples
Application information 
Courtesy Dr. Håkan Hedman, Umeå University, Sweden
Validation of anti-LRIG1 antibody AS184165 for immunohistochemistry. HEK293T cells were rendered LRIG1-null via CRISPR-Cas9 mediated ablation of the entire LRIG1 locus followed by transduction of the cells with a doxycycline-inducible LRIG1 allele encoding FLAG-tagged human LRIG1. LRIG1-expression was induced or not induced followed by pelleting of the cells, paraformaldehyde fixation, paraffin embedment, sectioning, and immunohistochemical staining using an automated Ventana staining machine and different concentrations of anti-LRIG1 or FLAG-M2 antibody. Shown are micrographs of LRIG1-induced cells (left, “Positive control”) and non-induced cells (right, “Negative control”). Anti-LRIG1 antibodies at 5 µg/ml.
Note that the cell population was heterogenous, with only a minority of the induced cells expressing FLAG-LRIG1.
Courtesy Dr. Håkan Hedman, Umeå University, Sweden
Additional information
Peptide used to elicit Lrig1 antibody is not conserved in Lrig3 protein.
IHC was performed on cell pellets made from LRIG1-null HEK293T cells with an inducible LRIG1 allele that had, or had not, been induced to express LRIG1. A specific signal was seen in the antibody range between 0.2 µg/ml and 5 µg/ml.Background
The LRIG1 protein Q96JA1 is coded by a gene on chromosome band 3p14.3, a region known to be deleted in various human cancers. This is considered to be a tumour suppressor gene in humans. LRIG1 is an integral cell-surface membrane protein, expressed by specific cells in various human tissues. Its 143-kDa form might be cleaved into 111-kDa and 32-kDa fragments. The LRIG1 protein may inhibit the growth of glial cell tumours and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumors.
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