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Med 6 | Mediator of RNA polymerase II transcription subunit 6

AS14 2802 |  Clonality: Polyclonal Host: Rabbit | Reactivity:Arabidopsis thaliana

Med 6 | Mediator of RNA polymerase II transcription subunit 6 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS14 2802)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known higher plant Med6 sequences, including Arabidopsis thaliana UniProt: F4IXJ7,  TAIR: AT3G21350

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1: 1000 (WB)
Expected | apparent MW

33.4 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples
western blot using anti-Med 6 antibodies

30 µg of total protein from Arabidopsis thaliana (leaves or seedlings tissues) extracted with extraction buffer (50 mM Tris/HCl pH7.5, 150mM NaCl, 5% glycerol, 1 mM EDTA, 5mM DTT, 0.5% (w/v) PVPP, 1% Protease inhibitors cocktail, 1% NP40) were separated on 10 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 min.

Courtesy of Dr. Ahmed Mohamed, School of Biological and Biomedical Sciences Durham University, United Kingdom

Additional information

Related products

Related products

AS14 2803 | anti-Med 7a/b | Mediator of RNA polymerase II transcription subunit 7a/b, rabbit antibodies

Antibodies involved in RNA metabolism and transcription regulation.

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background Med 6 (Mediator of RNA polymerase II transcription subunit 6) is a component of the Mediator complex which functions in transmition of information from gene-specific reulatory proteins to the basal RNA polymerase II transcription machinery.

Product citations

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