mLrig2-147 | Leucine-rich repeats and immunoglobulin-like domains protein 2

AS14 2788 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mice

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen form mice Lrig2 protein, UniProt: Q52KR2

Host Rabbit

Clonality Polyclonal

Purity Affinity purified IgG

Format Lyophilized in PBS pH 7.4

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (1 µg/ml)

Expected | apparent MW

117 | 140 kDa

Reactivity

Confirmed reactivity Mus musculus

Predicted reactivity

Mus musculus

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Thirteen µg of total protein from mouse embryonic fibroblasts (+/+, wild-type; +/-, heterozygous for Lrig2 exon 12; -/-, homozygously deficient of Lrig2 exon 12) extracted with lysis buffer containing 1% Triton X-100 were separated by electrophoresis on a 3 – 8% Tris-acetate NuPAGE gradient gel and blotted to a PVDF membrane. Blots were blocked with 5% nonfat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 over-night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10,000 for 1h at RT with agitation. The blot was washed as above and developed with ECL according to the manufacturer’s instructions.

 µg of total protein from LRIG1-transfected COS-7 cells extracted with lysis buffer (100mM Tris-HCl pH 7.5 1M, 300mM NaCl 5M, 2% Triton X-100) were separated on 3-8 % Tris acetate SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% dried milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody [1ug/uL] at a dilution of 1:3500, 1:1600, 1:800 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:5 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was  30 seconds.

Additional information

Antibody works on mouse embryonic fibroblast cell lysates from wild type vs. Lrig2 knockout cells.

Antibody reactivity was confirmed on mouse embryonic fibrobalsts.

Related products

AS18 4165 | Anti-LRIG1 | Lig-1, rabbit antibodies

AS06 156 | Anti-LRIG1 | Lig-1, chicken antibodies

AS14 2789 | Anti-mLrig3-207 | Leucine-rich repeats and immunoglobulin-like domains protein 3, rabbit antibodies

Background

Lrig2 (Leucine-rich repeats and immunoglobulin-like domains protein 2) is coded by Lrig2 gene and belongs to a family of integral membrane proteins. LIRG gene family is composed of three paralogues, LIRG1, LRIG2 and LRIG3.