mLrig3-207 | Leucine-rich repeats and immunoglobulin-like domains protein 3

AS14 2789 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mice

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from mice Lrig3 protein, UniProt: Q6P1C6

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 500 (3 µg/ml)

Expected | apparent MW

122 | 140 kDa

Reactivity

Confirmed reactivity Mus musculus

Predicted reactivity Bos taurus, Sus scrofa

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Thirty µg of total protein from mouse skin tissue (wild-type and Lrig3 knock-out mice) extracted with modified RIPA buffer were separated by electrophoresis on a 3 – 8% Tris-acetate NuPAGE gradient gel and blotted to a PVDF membrane. Blots were blocked with 5% nonfat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary Lrig3 antibody, upper part, at a dilution of 1:500 for 1h at RT with agitation (actin antibody, lower part). The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 for 1h at RT with agitation. The blot was washed as above and developed with ECL according to the manufacturer’s instructions.

Additional information

Antibody reactivity was confirmed on skin tissue lysates from wildtype vs, Lrig3 knockout mice

Background

Lrig3 (Leucine-rich repeats and immunoglobulin-like domains protein 3), involved in craniofacial and inner ear morphogenesis during embryonic development.

Product citations

Selected references Hellstrom et al. (2016). Cardiac hypertrophy and decreased high-density lipoprotein cholesterol in Lrig3-deficient mice. Am J Physiol Regul Integr Comp Physiol. 2016 Mar 23:ajpregu.00309.2015. doi: 10.1152/ajpregu.00309.2015.