Mouse IgG2a (Negative Control)
AS20 4451 | Clonality: Monoclonal | Host: Mouse | Reactivity: Mouse IgG2a
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Product Information
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG2a
Purity
Protein A/G purified
Format
Liquid in PBS with 0.08% sodium azide
Quantity
200 ug
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications
Flow Cytometry (Flowcyt)
Reactivity
Confirmed reactivity
Mouse IgG2a
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
For PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze.
Background
Background
Mouse IgG2a can be used as a negative control for immunofluorescence staining with FITC, Biotin and PE of mouse monoclonal antibodies. For estimation of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.
Product citations
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