mPGES-1 glutathione dependent prostaglandin E synthase

AS03 031 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human, Mouse, Sheep, Rat

Product Information

Immunogen

Recombinant human purified 6-His mPGES-1 O14684

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 10 000, human and rat samples (WB) 1 :6 000 on mice samples and in sheep seminal vesicles (IHC)

Expected | apparent MW

17 kDa

Reactivity

Confirmed reactivity Human, Mouse, Rat, sheep

Predicted reactivity Dog, Hamster, Horse

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Mus musculus (House mouse)

Application: Western Blotting

Pudmed ID: 28983247

Journal: Front Pharmacol

Figure Number: 1C

Published Date: 2017-10-07

First Author: Tuure, L., Hämäläinen, M., et al.

Impact Factor: 5.331

Open Publication

Dexamethasone inhibits mPGES-1 expression in activated macrophages in an MKP-1 dependent manner. (A) Effects of dexamethasone on peritoneal macrophages from wild-type and MKP-1 knock-out (KO) mice. Cells were incubated with LPS in the presence or absence of dexamethasone for 24 h. mPGES-1 mRNA levels were measured by quantitative RT-PCR and normalized against GAPDH mRNA levels. Results are expressed in arbitrary units, mPGES-1 mRNA levels in unstimulated cells from wild type mice were set as 1, and the other values were related to that. Results are expressed as mean + SEM, n = 4. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as ***P < 0.001 and ns = not significant. #P = 0.0286 vs unstimulated cells from wild-type mice. (B) Effect of dexamethasone on mPGES-1 mRNA production in J774 murine macrophages. Cells were stimulated with LPS in the presence or absence of dexamethasone for 24 h. mPGES-1 mRNA levels were measured by quantitative RT-PCR and normalized against GAPDH mRNA levels. Results are expressed in arbitrary units, mPGES-1 mRNA levels in LPS-stimulated cells were set as 100 % and the other values were related to that. Results are expressed as mean + SEM, n = 6–7. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as ***P < 0.001. (C) Effect of dexamethasone on mPGES-1 protein expression in J774 murine macrophages. Cells were stimulated with LPS in the presence or absence of dexamethasone for 24 h. mPGES-1 protein levels were measured by Western blot analysis and actin was used as a loading control. Results are expressed in arbitrary units, mPGES-1 protein levels in LPS-stimulated cells were set as 100% and the other values were related to that. Results are expressed as mean + SEM, n = 6. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as ***P < 0.001. Shown is a representative gel of six with similar results.

Reactant: Mus musculus (House mouse)

Application: Western Blotting

Pudmed ID: 28983247

Journal: Front Pharmacol

Figure Number: 6B

Published Date: 2017-10-07

First Author: Tuure, L., Hämäläinen, M., et al.

Impact Factor: 5.331

Open Publication

JNK inhibitor SP600125 inhibits the expression of mPGES-1 in activated macrophages. (A) Effects of a JNK inhibitor (SP600125), a p38 inhibitor (SB203580) and dexamethasone on mPGES-1 mRNA production in J774 murine macrophages. Cells were incubated with LPS and the compounds under investigation for 24 h. mPGES-1 mRNA levels were measured by quantitative RT-PCR and normalized against GAPDH mRNA levels. Results are expressed in arbitrary units, mPGES-1 mRNA levels in LPS-stimulated cells were set as 100% and the other values were related to that. Results are expressed as mean + SEM, n = 6–7. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as ***P < 0.001 and ns = not significant. (B) Effects of a JNK inhibitor (SP600125), a p38 inhibitor (SB203580) and dexamethasone on mPGES-1 protein expression in J774 murine macrophages. Cells were stimulated with LPS and the compounds under investigation for 24 h. mPGES-1 protein levels were measured by Western blot analysis and actin was used as a loading control. Results are expressed in arbitrary units, mPGES-1 protein levels in LPS-stimulated cells were set as 100% and the other values were related to that. Results are expressed as mean + SEM, n = 5–6. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as ***P < 0.001, **P < 0.01 and ns = not significant. Shown is a representative gel of five with similar results.

Reactant: Mus musculus (House mouse)

Application: Western Blotting

Pudmed ID: 29226622

Journal: Pharmacol Res Perspect

Figure Number: 1B

Published Date: 2017-12-01

First Author: Tuure, L., Hämäläinen, M., et al.

Impact Factor: None

Open Publication

Effects of the PDE4 inhibitor rolipram and dexamethasone on mPGES?1 expression in murine J774 macrophages. Cells were incubated with bacterial lipopolysaccharide (LPS) and rolipram or dexamethasone (which was used as a control compound) for 24 h. mPGES?1 mRNA levels were measured by quantitative RT?PCR and normalized against GAPDH mRNA levels (A). mPGES?1 protein expression was measured by Western blot where actin was used as a loading control (B). mPGES?1 expression levels in unstimulated (control) cells were set as 1 and the other values were related to that. Results are expressed as mean + SEM, n = 6–7 (A) and 5–6 (B). One?way ANOVA with Bonferroni's post?test was performed and statistical significance is indicated as **P < 0.01 and ***P < 0.001.

Reactant: Mus musculus (House mouse)

Application: Western Blotting

Pudmed ID: 29226622

Journal: Pharmacol Res Perspect

Figure Number: 4B

Published Date: 2017-12-01

First Author: Tuure, L., Hämäläinen, M., et al.

Impact Factor: None

Open Publication

Effects of the selective JNK inhibitor SP600125 and the selective p38 inhibitor BIRB796 on mPGES?1 expression in J774 murine macrophages. Cells were incubated with bacterial lipopolysaccharide (LPS) and SP600125 or BIRB796 for 24 h. mPGES?1 mRNA levels were measured by quantitative RT?PCR and normalized against GAPDH mRNA levels (A). mPGES?1 protein expression was measured by Western blot where actin was used as a loading control (B). mPGES?1 expression levels in the unstimulated (control) cells were set as 1 and the other values were related to that. Results are expressed as mean + SEM, n = 5–7. One?way ANOVA with Bonferroni's post?test was performed and statistical significance is indicated as **P < 0.01, ***P < 0.001 and ns = not significant.

Additional information

Additional information In our hands this antibody works with fresh frozen sections not in paraffin embeded material in IHCmPGES-1 antibody was characterized using spleen tissue from LPS-induced mPGES-1 knock-out and wild type mice, In spleen tissue from wt mice we observed strong positive staining in macrophage-like cells, while there was no staining for mPGES-1 in ko mice

Antibody is only recognizing mPGES1 not mPGES2 or cPGES.
Contains 0.1% ProClin.

Background

mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.

Product citations

Selected references Jiang et al (2021). Sonlicromanol's active metabolite KH176m normalizes prostate cancer stem cell mPGES-1 overexpression and inhibits cancer spheroid growth. PLoS One. 2021 Jul 9;16(7):e0254315. doi: 10.1371/journal.pone.0254315. PMID: 34242345; PMCID: PMC8270194.
Sadiba et al. (2021). Effects of a Novel GPR55 Antagonist on the Arachidonic AcidCascade in LPS-Activated Primary Microglial Cells. Int. J. Mol. Sci. 2021, 22, 2503.
Lio et al. (2019). Nardosinanone N suppresses LPS-induced macrophage activation by modulating the Nrf2 pathway and mPGES-1. Biochemical Pharmacology Sept 2019, 113639.
Tuure et al. (2019). Downregulation of microsomal prostaglandin E synthase-1 (mPGES-1) expression in chondrocytes is regulated by MAP kinase phosphatase-1 (MKP-1). Int Immunopharmacol. 2019 Mar 18;71:139-143. doi: 10.1016/j.intimp.2019.03.014.
Gargouri et al. (2018: Anti-neuroinflammatory effects of Ginkgo biloba extract EGb761 in LPS-activated primary microglial cells. Phytomedicine, doi.org/10.1016/j.phymed.2018.04.009
Tuure et al. (2017). PDE4 inhibitor rolipram inhibits the expression of microsomal prostaglandin E synthase-1 by a mechanism dependent on MAP kinase phosphatase-1. Pharmacol Res Perspect. 2017 Dec;5(6). doi: 10.1002/prp2.363. Pharmacol Res Perspect. 2017 Dec;5(6). doi: 10.1002/prp2.363.
Kern et al. (2017). CD200 selectively upregulates prostaglandin E2 and D2 synthesis in LPS-treated bone marrow-derived macrophages. Prostaglandins Other Lipid Mediat. 2017 Jun 3. pii: S1098-8823(17)30010-2. doi: 10.1016/j.prostaglandins.2017.06.002.
Bhatia et al. (2017). Alleviation of Microglial Activation Induced by p38 MAPK/MK2/PGE2 Axis by Capsaicin: Potential Involvement of other than TRPV1 Mechanism/s. Sci Rep. 2017 Dec;7(1):116. doi: 10.1038/s41598-017-00225-5.
Tuure et al. (2014). Aurothiomalate inhibits the expression of mPGES-1 in primary human chondrocytes. Scand J Rheumatol. 2014 Oct 14:1-6.
Olajide et al. (2014). Picralima nitida seeds suppress PGE2 production by interfering with multiple signalling pathways in IL-1?-stimulated SK-N-SH neuronal cells. J Ethnopharmacol. 2014 Jan 31. pii: S0378-8741(14)00074-9. doi: 10.1016/j.jep.2014.01.027.

calculated \| apparent molecular mass [kDa]:	17 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant human purified 6-His mPGES-1 O14684
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 10 000, human and rat samples (WB) 1 :6 000 on mice samples and in sheep seminal vesicles (IHC)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunohistochemistry (IHC), Western blot (WB)
background:	mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.
All references:	Jiang et al (2021). Sonlicromanol's active metabolite KH176m normalizes prostate cancer stem cell mPGES-1 overexpression and inhibits cancer spheroid growth. PLoS One. 2021 Jul 9;16(7):e0254315. doi: 10.1371/journal.pone.0254315. PMID: 34242345; PMCID: PMC8270194. Sadiba et al. (2021). Effects of a Novel GPR55 Antagonist on the Arachidonic AcidCascade in LPS-Activated Primary Microglial Cells. Int. J. Mol. Sci. 2021, 22, 2503. Lio et al. (2019). Nardosinanone N suppresses LPS-induced macrophage activation by modulating the Nrf2 pathway and mPGES-1. Biochemical Pharmacology Sept 2019, 113639. Tuure et al. (2019). Downregulation of microsomal prostaglandin E synthase-1 (mPGES-1) expression in chondrocytes is regulated by MAP kinase phosphatase-1 (MKP-1). Int Immunopharmacol. 2019 Mar 18;71:139-143. doi: 10.1016/j.intimp.2019.03.014. Gargouri et al. (2018: Anti-neuroinflammatory effects of Ginkgo biloba extract EGb761 in LPS-activated primary microglial cells. Phytomedicine, doi.org/10.1016/j.phymed.2018.04.009 Tuure et al. (2017). PDE4 inhibitor rolipram inhibits the expression of microsomal prostaglandin E synthase-1 by a mechanism dependent on MAP kinase phosphatase-1. Pharmacol Res Perspect. 2017 Dec;5(6). doi: 10.1002/prp2.363. Pharmacol Res Perspect. 2017 Dec;5(6). doi: 10.1002/prp2.363. Kern et al. (2017). CD200 selectively upregulates prostaglandin E2 and D2 synthesis in LPS-treated bone marrow-derived macrophages. Prostaglandins Other Lipid Mediat. 2017 Jun 3. pii: S1098-8823(17)30010-2. doi: 10.1016/j.prostaglandins.2017.06.002. Bhatia et al. (2017). Alleviation of Microglial Activation Induced by p38 MAPK/MK2/PGE2 Axis by Capsaicin: Potential Involvement of other than TRPV1 Mechanism/s. Sci Rep. 2017 Dec;7(1):116. doi: 10.1038/s41598-017-00225-5. Tuure et al. (2014). Aurothiomalate inhibits the expression of mPGES-1 in primary human chondrocytes. Scand J Rheumatol. 2014 Oct 14:1-6. Olajide et al. (2014). Picralima nitida seeds suppress PGE2 production by interfering with multiple signalling pathways in IL-1?-stimulated SK-N-SH neuronal cells. J Ethnopharmacol. 2014 Jan 31. pii: S0378-8741(14)00074-9. doi: 10.1016/j.jep.2014.01.027.
additional information:	In our hands this antibody works with fresh frozen sections not in paraffin embeded material in IHCmPGES-1 antibody was characterized using spleen tissue from LPS-induced mPGES-1 knock-out and wild type mice, In spleen tissue from wt mice we observed strong positive staining in macrophage-like cells, while there was no staining for mPGES-1 in ko mice
additional information (application):	Antibody is only recognizing mPGES1 not mPGES2 or cPGES. Contains 0.1% ProClin.
Confirmed reactivity:	Human, Mouse, Rat, sheep
predicted reactivity:	Dog, Hamster, Horse
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
More images:	Reactant: Mus musculus (House mouse) Application: Western Blotting Pudmed ID: 28983247 Journal: Front Pharmacol Figure Number: 1C Published Date: 2017-10-07 First Author: Tuure, L., Hämäläinen, M., et al. Impact Factor: 5.331 Open Publication Dexamethasone inhibits mPGES-1 expression in activated macrophages in an MKP-1 dependent manner. (A) Effects of dexamethasone on peritoneal macrophages from wild-type and MKP-1 knock-out (KO) mice. Cells were incubated with LPS in the presence or absence of dexamethasone for 24 h. mPGES-1 mRNA levels were measured by quantitative RT-PCR and normalized against GAPDH mRNA levels. Results are expressed in arbitrary units, mPGES-1 mRNA levels in unstimulated cells from wild type mice were set as 1, and the other values were related to that. Results are expressed as mean + SEM, n = 4. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as *P < 0.001 and ns = not significant. #P = 0.0286 vs unstimulated cells from wild-type mice. (B) Effect of dexamethasone on mPGES-1 mRNA production in J774 murine macrophages. Cells were stimulated with LPS in the presence or absence of dexamethasone for 24 h. mPGES-1 mRNA levels were measured by quantitative RT-PCR and normalized against GAPDH mRNA levels. Results are expressed in arbitrary units, mPGES-1 mRNA levels in LPS-stimulated cells were set as 100 % and the other values were related to that. Results are expressed as mean + SEM, n = 6–7. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as P < 0.001. (C) Effect of dexamethasone on mPGES-1 protein expression in J774 murine macrophages. Cells were stimulated with LPS in the presence or absence of dexamethasone for 24 h. mPGES-1 protein levels were measured by Western blot analysis and actin was used as a loading control. Results are expressed in arbitrary units, mPGES-1 protein levels in LPS-stimulated cells were set as 100% and the other values were related to that. Results are expressed as mean + SEM, n = 6. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as P < 0.001. Shown is a representative gel of six with similar results. Reactant: Mus musculus (House mouse) Application: Western Blotting Pudmed ID: 28983247 Journal: Front Pharmacol Figure Number: 6B Published Date: 2017-10-07 First Author: Tuure, L., Hämäläinen, M., et al. Impact Factor: 5.331 Open Publication JNK inhibitor SP600125 inhibits the expression of mPGES-1 in activated macrophages. (A) Effects of a JNK inhibitor (SP600125), a p38 inhibitor (SB203580) and dexamethasone on mPGES-1 mRNA production in J774 murine macrophages. Cells were incubated with LPS and the compounds under investigation for 24 h. mPGES-1 mRNA levels were measured by quantitative RT-PCR and normalized against GAPDH mRNA levels. Results are expressed in arbitrary units, mPGES-1 mRNA levels in LPS-stimulated cells were set as 100% and the other values were related to that. Results are expressed as mean + SEM, n = 6–7. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as P < 0.001 and ns = not significant. (B) Effects of a JNK inhibitor (SP600125), a p38 inhibitor (SB203580) and dexamethasone on mPGES-1 protein expression in J774 murine macrophages. Cells were stimulated with LPS and the compounds under investigation for 24 h. mPGES-1 protein levels were measured by Western blot analysis and actin was used as a loading control. Results are expressed in arbitrary units, mPGES-1 protein levels in LPS-stimulated cells were set as 100% and the other values were related to that. Results are expressed as mean + SEM, n = 5–6. One-way ANOVA with Bonferroni's post-test was performed and statistical significance is indicated as P < 0.001, P < 0.01 and ns = not significant. Shown is a representative gel of five with similar results. Reactant: Mus musculus (House mouse) Application: Western Blotting Pudmed ID: 29226622 Journal: Pharmacol Res Perspect Figure Number: 1B Published Date: 2017-12-01 First Author: Tuure, L., Hämäläinen, M., et al. Impact Factor: None Open Publication Effects of the PDE4 inhibitor rolipram and dexamethasone on mPGES?1 expression in murine J774 macrophages. Cells were incubated with bacterial lipopolysaccharide (LPS) and rolipram or dexamethasone (which was used as a control compound) for 24 h. mPGES?1 mRNA levels were measured by quantitative RT?PCR and normalized against GAPDH mRNA levels (A). mPGES?1 protein expression was measured by Western blot where actin was used as a loading control (B). mPGES?1 expression levels in unstimulated (control) cells were set as 1 and the other values were related to that. Results are expressed as mean + SEM, n = 6–7 (A) and 5–6 (B). One?way ANOVA with Bonferroni's post?test was performed and statistical significance is indicated as P < 0.01 and P < 0.001. Reactant: Mus musculus (House mouse)* Application: Western Blotting Pudmed ID: 29226622 Journal: Pharmacol Res Perspect Figure Number: 4B Published Date: 2017-12-01 First Author: Tuure, L., Hämäläinen, M., et al. Impact Factor: None Open Publication Effects of the selective JNK inhibitor SP600125 and the selective p38 inhibitor BIRB796 on mPGES?1 expression in J774 murine macrophages. Cells were incubated with bacterial lipopolysaccharide (LPS) and SP600125 or BIRB796 for 24 h. mPGES?1 mRNA levels were measured by quantitative RT?PCR and normalized against GAPDH mRNA levels (A). mPGES?1 protein expression was measured by Western blot where actin was used as a loading control (B). mPGES?1 expression levels in the unstimulated (control) cells were set as 1 and the other values were related to that. Results are expressed as mean + SEM, n = 5–7. One?way ANOVA with Bonferroni's post?test was performed and statistical significance is indicated as P < 0.01, *P < 0.001 and ns = not significant.

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