Name:
Phone:
E-mail:
Message


a

mPGES-1 glutathione dependent prostaglandin E synthase

AS03 031 | Clonality: Polyclonal  | Host: Rabbit | Reactivity: Human, Mouse, Sheep, Rat

mPGES-1 glutathione dependent prostaglandin E synthase  in the group Human/Animal Antibodies / Human Proteins / Other Human proteins at Agrisera AB (Antibodies for research) (AS03 031)

PRODUCT INFORMATION IN PDF

Qty: 
272 €
Buy 2 items of this product for 202 €/items
Buy 3 items of this product for 185 €/items

Datasheet Product citations Add review

Product Information

Immunogen

Recombinant human purified 6-His mPGES-1 O14684

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 10 000, human and rat samples (WB) 1 :6 000 on mice samples and in sheep seminal vesicles (IHC)
Expected | apparent MW

17 kDa

Reactivity

Confirmed reactivity Human, Mouse, Rat, sheep
Predicted reactivity Dog, Hamster, Horse
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information In our hands this antibody works with fresh frozen sections not in paraffin embeded material in IHCmPGES-1 antibody was characterized using spleen tissue from LPS-induced mPGES-1 knock-out and wild type mice. In spleen tissue from wt mice we observed strong positive staining in macrophage-like cells, while there was no staining for mPGES-1 in ko mice.
Antibody is only recognizing mPGES1 not mPGES2 or cPGES.
This product can be sold containing proclin if requested.

Related products

Related products

AS03 031S | mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control

Secondary antibodies

Background

Background

mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.

Product citations

Selected references Gargouri et al. (2018: Anti-neuroinflammatory effects of Ginkgo biloba extract EGb761 in LPS-activated primary microglial cells. Phytomedicine, doi.org/10.1016/j.phymed.2018.04.009
Tuure et al. (2017). PDE4 inhibitor rolipram inhibits the expression of microsomal prostaglandin E synthase-1 by a mechanism dependent on MAP kinase phosphatase-1. Pharmacol Res Perspect. 2017 Dec;5(6). doi: 10.1002/prp2.363. Pharmacol Res Perspect. 2017 Dec;5(6). doi: 10.1002/prp2.363.
Kern et al. (2017). CD200 selectively upregulates prostaglandin E2 and D2 synthesis in LPS-treated bone marrow-derived macrophages. Prostaglandins Other Lipid Mediat. 2017 Jun 3. pii: S1098-8823(17)30010-2. doi: 10.1016/j.prostaglandins.2017.06.002.
Bhatia et al. (2017). Alleviation of Microglial Activation Induced by p38 MAPK/MK2/PGE2 Axis by Capsaicin: Potential Involvement of other than TRPV1 Mechanism/s. Sci Rep. 2017 Dec;7(1):116. doi: 10.1038/s41598-017-00225-5.
Tuure et al. (2014). Aurothiomalate inhibits the expression of mPGES-1 in primary human chondrocytes. Scand J Rheumatol. 2014 Oct 14:1-6.
Olajide et al. (2014). Picralima nitida seeds suppress PGE2 production by interfering with multiple signalling pathways in IL-1β-stimulated SK-N-SH neuronal cells. J Ethnopharmacol. 2014 Jan 31. pii: S0378-8741(14)00074-9. doi: 10.1016/j.jep.2014.01.027.