mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control

Positive control: 5 μl load per well is optimal for most chemiluminescent detection systems.

Preabsorption:  6-12 ug of protein/100ul diluted anti-mPGES-1 serum (1:5000)

16-17 kDa

Protein is provided in 10 mM NaPI, pH 8, 150 mM KCl, 0.1 mM EDTA, 1mM GSH, 10 % glycerol, 0,1 % reduced Triton X-100. It has been produced in E.coli.

If intended to be used in Western blot, loading buffer should be added and the solution should be heated up in 98°C for 5 minutes before loading on the gel.

Concentration:  0.42 µg/µl.

Western blot: recommended load per gel to allow Coomasie staining and western blot detection is 5 µl

Immunolocalization: 6-12 ug of protein/100 µl diluted anti-mPGES-1 serum (1:5000) was used for preabsorption of mPGES-1 antibodies followed by immunolocalization.

mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.