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AGO set
Tag Antibody Set 

mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control

AS03 031S  |  Recombinant protein | Positive control

mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control in the group Antibodies for Human/Animal  / Human Proteins / Other Human proteins at Agrisera AB (Antibodies for research) (AS03 031S)

PRODUCT INFORMATION IN PDF

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182 €

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Product Information

Format Liquid
Quantity 50 µl (21 µg)
Storage Store protein standard at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (blocking protein for antibody), Western blot (WB)
Recommended dilution

Positive control: 5 μl load per well is optimal for most chemiluminescent detection systems.

Preabsorption:  6-12 ug of protein/100ul diluted anti-mPGES-1 serum (1:5000)

Expected | apparent MW

16-17 kDa

Reactivity

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Protein is provided in 10 mM NaPI, pH 8, 150 mM KCl, 0.1 mM EDTA, 1mM GSH, 10 % glycerol, 0,1 % reduced Triton X-100. It has been produced in E.coli.

If intended to be used in Western blot, loading buffer should be added and the solution should be heated up in 98°C for 5 minutes before loading on the gel.

Concentration:  0.42 µg/µl.

Western blot: recommended load per gel to allow Coomasie staining and western blot detection is 5 µl

Immunolocalization: 6-12 ug of protein/100 µl diluted anti-mPGES-1 serum (1:5000) was used for preabsorption of mPGES-1 antibodies followed by immunolocalization.

Related products

Related products

AS03 031 | Anti-mPGES-1 glutathione dependent prostaglandin E synthase, rabbit antibodies

Background

Background

mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.

Product citations

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