NAD3 | NADH-ubiquinone oxidoreductase chain 3

AS16 3831 | Clonality: Polyclonal | Host: Rabbit | ractivity: Oryza sativa

Product Information

Immunogen KLH-conjugated synthetic peptide chosen from Oryza sativa NAD3 protein sequence UniProt: Q7GHI8; LOC_Osm1g00280.1

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 2 x 50 µl

Reconstitution For reconstitution add 50 µl of sterile water to each tube.

Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles.

Tested applications Western blot (WB)

Recommended dilution 1: 1000 (WB)

Expected | apparent MW

17 kDa

Reactivity

Confirmed reactivity Oryza sativa

Predicted reactivity Zea mays
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Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

50-100 µg of total protein from Oryza sativa flag leaf, roots or fringe extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 -1:1000 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min. and 3 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP/conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min. with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

Additional information

Related products

Related products AS15 2926 | Anti-NAD6 | NADH-ubiquinone oxidoreductase chain 6, rabbit antibodies
collection of antibodies to mitochondrial proteins

Background

Background NAD3 (NADH dehydrogenase subunit 3) is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It functions in the transfer of electrons from NADH to the respiratory chain.