NAD3 | NADH-ubiquinone oxidoreductase chain 3
AS16 3831 | Clonality: Polyclonal | Host: Rabbit | ractivity: Oryza sativa
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Product Information
Immunogen
KLH-conjugated synthetic peptide chosen from Oryza sativa NAD3 protein sequence UniProt: Q7GHI8; LOC_Osm1g00280.1
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
2 x 50 µl
Reconstitution
For reconstitution add 50 µl of sterile water to each tube.
Storage
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Tested applications
Western blot (WB)
Recommended dilution
1: 1000 (WB)
Expected | apparent MW
17 kDa
Reactivity
Confirmed reactivity
Oryza sativa
Predicted reactivity
Zea mays
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example
50-100 µg of total protein from Oryza sativa flag leaf, roots or fringe extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 -1:1000 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min. and 3 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP/conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min. with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.
50-100 µg of total protein from Oryza sativa flag leaf, roots or fringe extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 -1:1000 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min. and 3 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP/conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min. with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.
Additional information
Background
Background
NAD3 (NADH dehydrogenase subunit 3) is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It functions in the transfer of electrons from NADH to the respiratory chain.
Product citations
Selected references
Chen et al. (2019). Composition of Mitochondrial Complex I during the Critical Node of Seed Aging in Oryza sativa. Journal of Plant Physiology Volume 236, May 2019, Pages 7-14.
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