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NdhF | NAD(P)H dehydrogenase subunit 5 (chloroplastic)
AS13 2711 | Clonality: Polyclonal | Host: Rabbit | Reactivity:
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Oryza sativa OsChl78 UniProt: P0C328
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 ”l Reconstitution: For reconstitution add 50 µl of sterile water Storage: The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 83 | 58 kDa
- Reactivity
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Confirmed reactivity: Oryza sativa Not reactive in: Zea mays - Application Examples
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Application example: 
Total protein from Oryza sativa rice (CV. 9311) flag leaf at the tillering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer [62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12,000 rpm for 10min at 4 ℃, and the supernatant was collected and stored at –70 ℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF.
Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 120 seconds. - Additional Information
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Additional information (application): This product can be sold containing proclin if requested - Background
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Background: NDH (NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names: NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic, NADH-plastoquinone oxidoreductase subunit 5.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
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Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S
This product can be purchased in 3 different volumes:
AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer
AS16 ECL-SN, 100 ml
AS16 ECL-SN-1L, 1L
Choose the appropriate volume in the drop down menu to the right
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
AS16 ECL-S-1L, 1L
Choose the appropriate volume in the drop down menu to the right