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NdhF | NAD(P)H dehydrogenase subunit 5 (chloroplastic)

AS13 2711 |  Clonality: Polyclonal | Host: Rabbit | Reactivity:

NdhF | NAD(P)H dehydrogenase subunit 5 (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS13 2711)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Oryza sativa OsChl78 UniProt: P0C328

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage The antibody may be stored at -20℃ for one year in its original formulation. Additionally, antibody may be stored at 2℃ to 8℃ for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

83 | 58 kDa

Reactivity

Confirmed reactivity
Not reactive in Zea mays

Application examples

Application examples Application example:



Total protein from Oryza sativa rice (CV. 9311) flag leaf at the tillering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer [62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12,000 rpm for 10min at 4 ℃, and the supernatant was collected and stored at –70 ℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF.
Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 120 seconds.

Additional information

This product can be sold containing proclin if requested.

Related products

Related products AS16 4064 | Anti-NdhB | NAD(P)H-quinone oxidoreductase subunit 2 (chloroplastic), rabbit antibodies
AS13 2711 | Anti-NdhF | NAD(P)H dehydrogenase subunit 5 (chloroplastic), rabbit antibodies
AS16 4065 | Anti-NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic), rabbit antibodies
AS16 4066 | Anti-NdhS | NAD(P)H-quinone oxidoreductase subunit S (chloroplastic), rabbit antibodies 
antibody collection to proteins involved in photosynthetic electron transfer

Background

Background

NDH (NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur  centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain.  Alternative names: NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic, NADH-plastoquinone oxidoreductase subunit 5.

Product citations

Selected references

to be added when available, antibody released in March 2015.

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