Name:
Phone:
E-mail:
Message


 


NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic)

AS13 2712 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Limited stock.

NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS13 2712)

DATA SHEET IN PDF

Qty: 
200
Buy 2 items of this product for 173.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Oryza sativa OsChl78UniProt: P0C337

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage The antibody may be stored at -20℃ for one year in its original formulation. Additionally, antibody may be stored at 2℃ to 8℃ for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

40-60 kDa (2 bands)

Reactivity

Confirmed reactivity
Predicted reactivity Hordeum vulgare
Species of your interest not listed? Contact us

Application examples

Application examples application example


western blot using anti-NAD(P)H-quinone oxidoreductase subunit H, chloroplastic  antibodies

Total protein from Oryza sativa rice (CV. 9311) flag leaf at the tillering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer [62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12,000 rpm for 10min at 4 ℃, and the supernatant was collected and stored at –70 ℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF.
Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 3 min

Additional information

This product can be sold containing proclin if requested.

Related products

Related products AS16 4064 | Anti-NdhB | NAD(P)H-quinone oxidoreductase subunit 2 (chloroplastic), rabbit antibodies
AS13 2711 | Anti-NdhF | NAD(P)H dehydrogenase subunit 5 (chloroplastic), rabbit antibodies
AS16 4065 | Anti-NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic), rabbit antibodies (Arabidopsis thaliana)
AS16 4066 | Anti-NdhS | NAD(P)H-quinone oxidoreductase subunit S (chloroplastic), rabbit antibodies 

antibody collection to proteins involved in photosynthetic electron transfer

Secondary antibodies

Background

Background

NAD(P)H dehydrogenase subunit H (EC=1.6.5) is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur  centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names:  NAD(P)H dehydrogenase subunit H; NDH7
NADH-plastoquinone oxidoreductase 49 kDa subunit, NADH-plastoquinone oxidoreductase subunit H.

Product citations

Selected references Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.

Related products: NdhH | NAD(P)H-quinone oxidoreductase subunit H (chloroplastic)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €
AS16 4064 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea...
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS16 4065 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items