Native mouse beta NGF purified from submaxillary salivary gland,
Total IgG. Protein G purified.
For reconstitution add 500 ĩl of sterile water
After reconstitution keep aliquots at -20šC for a higher stability. and at 4šC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Immunohistochemistry (IHC), 1-site ELISA (ELISA), Western blot (WB), immunoblot,
A concentration of 1-3 ĩg/ml is recommended for IHC, western blot and immunoblot, and ELISA, Use neat for in vivo studies at 2-10 ĩg/ml (ED50), This antibody was tested on cultured sensory neurons supported by 100ng/ml of purified mouse beta NGF, Be advised that 2ug/ml will neutralize 100ng/ml of mouse NGF, The higher 10ug/ml is only recommended if the concentration of NGF being used is higher than 100ng/ml such as the 200 or 500ng/ml that is occasionally used in some culture systems, This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro, Optimal dilutions is determined by the end user