Non-fucosylated xyloglucan-3 (clone CCRC-M100)
AS16 3234 | Clonality: monoclonal | Host: Mouse | Reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum, Tamarindus indicus
From the laboratory of Michael G. Hahn, PhD, University of Georgia
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
BSA-conjugated sycamore ((Acer pseudoplatanus)) xyloglucan, covalent binding.
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgM
Format
Cell culture supernatant, liquid.
Quantity
5 ml
Storage
Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year.
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution
Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
Reactivity
Confirmed reactivity
Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum, Tamarindus indicus
Predicted reactivity
Dicots
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Exact working dilution needs to be determined by end user.
CCRC-M100 binds only to the xyloglucan sub-unit, XXXG, and shows no cross-reactivity with other xyloglucan sub-units tested. CCRC-M100 shows some cross-reactivity with sycamore pectic polysaccharides and linseed mucilage.
Background
Background
Xyloglucans are polysaccharides commonly refered to as hemicelluloses found in the primary cell walls of vascular plants. This antibody binds to galactosylated side-chains of non-fucosylated xyloglucan-3, and appears to preferentially bind to the galactosylated side-chain closest to the reducing end of xyloglucan oligosaccharide sub-units (XXXG).
Product citations
Selected references
Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
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