NpHR | Halorhodopsin blocking peptide
AS12 1851P | Blocking peptide
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Estimation of the ratio between antibodies and a peptide : In neutralisation assay use 0,73 µl of Halorhodopsin peptide for 10 ml of reaction volume.
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This blocking peptide can be used as a control to neutralize Halorhodopsin before immunolocalization or western blot. Furter details are provided below.
Halorhodopsin (HR) is a hyperpolarizing light-driven ion pump from the halophilic archaea bacterium Natronomonas pharaonis. HR uses the energy of yellow light (excitation maximum near 580 nm) to mediate primarily chloride but also bromide, iodide, and nitrate import into the cell against their electrochemical gradients.
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