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Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)

AS18 4197  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses
Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5) in the group Antibodies for Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4197)

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen Pectic polysaccharide (beta 1,4-galactan)
Host Rat
Clonality Monoclonal
Subclass/isotype IgG
Format Cell Culture Supernatant
Quantity 5 ml
Storage Store at +4°C (short term) and at -20°C (long term).

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide.

No cross-reactivity with (1-3)-beta-D-galactans or (1-6)-beta-D-galactans.
It recognizes a linear tetrasaccharide in (1-4)-beta-D-galactans.
In ELISA (competitive inhibition), antibody is binding to: (1-4)-beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-beta-D-galactan.

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Background

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.  

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

Product citations

Selected references Andersen et al. (2016). Characterization of the LM5 pectic galactan epitope with synthetic analogues of ß-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec ;436:36-40.doi: 10.1016/j.carres.2016.10.012.
Jones et al. (1997). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3.

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