Pectic polysaccharide, alpha-1,5-arabinan (monoclonal, clone LM6)
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Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
No cross-reactivity with gum arabic. The antibody recognises a linear pentasaccharide in (1-5)-α-L-arabinans. I many species it can also recognise pectic polysaccharides.
In some species this antibody could recognize arabinogalactan-proteins (AGPs).
In competitive inhibition ELISAs, antibody is binding to: (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabinohexaose.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.
Willats et el. (1998). Generation of monoclonal antibody specific to (1-->5)-alpha-L-arabinan. Carbohydr Res. 1998 Mar;308(1-2):149-52.doi: 0.1016/s0008-6215(98)00070-6.