Pectic polysaccharide, alpha-1,5-arabinan (monoclonal, clone LM6)
AS18 4198 | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

Data sheet | Product citations | Add review |
Product Information
Immunogen
Pectic polysaccharide (alpha-1,5-arabinan), neoglycoprotein (arabinoheptaose-BSA conjugated).
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgG
Format
Cell Culture Supernatant
Quantity
5ml
Storage
Store at +4°C (short term) and at -20°C (long term).
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses.
Application examples
Additional information
Additional information
Contains 0.05% Sodium Azide
No cross-reactivity with gum arabic. The antibody recognises a linear pentasaccharide in (1-5)-α-L-arabinans. I many species it can also recognise pectic polysaccharides.
In some species this antibody could recognize arabinogalactan-proteins (AGPs).
In competitive inhibition ELISAs, antibody is binding to: (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabinohexaose.
No cross-reactivity with gum arabic. The antibody recognises a linear pentasaccharide in (1-5)-α-L-arabinans. I many species it can also recognise pectic polysaccharides.
In some species this antibody could recognize arabinogalactan-proteins (AGPs).
In competitive inhibition ELISAs, antibody is binding to: (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabinohexaose.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.
Product citations
Selected references
Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.
Willats et el. (1998). Generation of monoclonal antibody specific to (1-->5)-alpha-L-arabinan. Carbohydr Res. 1998 Mar;308(1-2):149-52.doi: 0.1016/s0008-6215(98)00070-6.
Willats et el. (1998). Generation of monoclonal antibody specific to (1-->5)-alpha-L-arabinan. Carbohydr Res. 1998 Mar;308(1-2):149-52.doi: 0.1016/s0008-6215(98)00070-6.
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