Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7)
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Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Has no known cross-reactivity with other polymers.
Binds to methyl esterified homogalacturonan.
Does not bind to un-esterified homogalacturonan.
This antibody is a good marker for pectic homogalacturonan.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.
Knox et al. (1990). Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta. 1990 Jul;181(4):512-21.doi: 0.1007/BF00193004.