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Pectic polysaccharide, homogalacturonan (monoclonal, clone LM19)

AS18 4191  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses
Pectic polysaccharide, homogalacturonan (monoclonal, clone LM19) in the group Antibodies for Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4191)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen Pectic polysaccharide, Homogalacturonan.
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Format Cell Culture Supernatant
Quantity 5 ml
Storage Store at +4°C (short term) and at -20°C (long term).

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Application examples

Immunfluorescence method described in:
An extended set of monoclonal antibodies to pectic homogalacturonan. Yves Verhertbruggen, Susan E. Marcus, Ash Haeger, José J. Ordaz-Ortiz, J. Paul Knox, Carbohydrate research 344 (2009) 1858-1862.

Additional information

Additional information Contains 0.05% Sodium Azide.

Has no known cross-reactivity with other polymers.
Binds to unesterified homogalacturonan.

The antibody recognizes a range of homogalacturonan samples but binds strongly to un-esterified homogalacturonan.

Related products

Background

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.  

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

Product citations

Selected references Marcus et al. (2010). Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct;64(2):191-203.doi: 0.1111/j.1365-313X.2010.04319.x.
Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.

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