Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)
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Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes.
The antibody does not bind to un-estrified homogalacuronan.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls
Willats et al. (2001). Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl sterase action, matrix properties, and cell adhesion. j. Biiol Chem. 2001 Jun 1;276(22):19404-13.doi: 10.1074/jbc.M011242200.