Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)
AS18 4196 | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
Pectic polysaccharide, Homogalacturonan.
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgM
Format
Cell Culture Supernatant
Quantity
5 ml
Storage
Store at +4°C (short term) and at -20°C (long term).
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses.
Not reactive in
No confirmed exceptions from predicted reactivity known at the moment.
Application examples
Additional information
Additional information
Contains 0.05% Sodium Azide
The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes.
The antibody does not bind to un-estrified homogalacuronan.
The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes.
The antibody does not bind to un-estrified homogalacuronan.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell wallsProduct citations
Selected references
Clausen et al. (2003). Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 003 Aug 12;338(17):1797-800.doi: 10.1016/s0008-6215(03)00272-6.
Willats et al. (2001). Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl sterase action, matrix properties, and cell adhesion. j. Biiol Chem. 2001 Jun 1;276(22):19404-13.doi: 10.1074/jbc.M011242200.
Willats et al. (2001). Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl sterase action, matrix properties, and cell adhesion. j. Biiol Chem. 2001 Jun 1;276(22):19404-13.doi: 10.1074/jbc.M011242200.
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