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Pectic polysaccharide, Rhamnogalacturonan (monoclonal, clone LM16)

AS18 4201  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses
Pectic polysaccharide, Rhamnogalacturonan (monoclonal, clone LM16) in the group Antibodies for Plant/Algal  / Cell Wall at Agrisera AB (Antibodies for research) (AS18 4201)

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Product Information

Immunogen Pectic polysaccharide, Rhamnogalacturonan
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Format Cell culture supernatant
Quantity 5 ml
Storage Store at +4°C (short term) and at -20°C (long term).
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Application examples

Immunofluorescent visualization of rhamnogalacturonan in Nicotiana tabacum stem.
Applied immunolocalization method was as described in Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.

Additional information

Additional information Contains 0.05% Sodium Azide.

Reacts with polysaccharide, rhamnogalacturonan-I (RG-I) The binding could be sensitive to galactosidase action and the epitope could involve galactosyl residue(s) on the rhamnogalacturonan backbones.

Recognizes a epitope associated with arabinans and can be generated by arabinofuranosidase action and the loss of arabinosyl residues.

Related products

Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.

Product citations

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