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Peptostreptococcus magnus Protein L

IMS02-136-331  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Peptostreptococcus magnus

Peptostreptococcus magnus Protein L  in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (IMS02-136-331)

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Product Information

Immunogen

Purified, full length recombinant protein

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY
Format Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Quantity 100 µl (1 mg/ml)
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Affinity purified IgY
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

36 kDa

Reactivity

Confirmed reactivity Peptostreptococcus magnus
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Related products

Related products

IMS02-008-333 | Anti-Staphylococcal Protein A, chicken antibodies

IMS02-109-334 | Anti-Streptococcal Protein G, chicken antibodies

Secondary antibodies

Background

Background

Protein L is a 36 kDa immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L. Protein L binding is restricted to those antibodies that contain kappa light chains and it is only effective in binding certain subtypes of kappa light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLkappa-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.

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