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Phy | Phytochrome (clone Oat-23)

AS20 4505 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avena sativa, Pisum sativum
Phy | Phytochrome (clone Oat-23) in the group Antibodies for Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS20 4505)

DATA SHEET IN PDF

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320
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Phytochrome
Host Mouse
Clonality Monoclonal
Format Cell culture supernatant
Quantity 5 ml
Storage Store at -80°C; Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Competitive ELISA, Immunoflourescence (IF), Immunoprecipiation (IP), Western blot (WB)
Recommended dilution assay dependent
Expected | apparent MW 124 kDa

Reactivity

Confirmed reactivity Avena sativa, Pisum sativum
Predicted reactivity graminae, fabaceae
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Additional information

Epitope for this antibody is located at 36 kDa from N-terminus and very near the site of chromophore attachment.

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Background

Background Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.

Product citations

Selected references Pratt et al. (1988). Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides. Arch Biochem Biophys. 267(2):723-35. doi: 10.1016/0003-9861(88)90081-1. Cordonnier et al. (1983). Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta. 158(4):369-76. doi: 10.1007/BF00397340.

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