showForm Anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3 antibodies

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PIP1;3 | Aquaporin, plasma membrane intrinistic protein 1-3

AS09 504 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa, Zea mays

PIP1;3 | Aquaporin, plasma membrane intrinistic protein 1-3 in the group Antibodies for Plant/Algal  / Membrane Transport System / Plasma membrane at Agrisera AB (Antibodies for research) (AS09 504)

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Product Information

Immunogen

Synthetic peptide derived from N-terminal part of Oryza sativa PIP1-3 Q9SXF8

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

30.7 | 26-29 kDa (Oryza sativa)

Reactivity

Confirmed reactivity Oryza sativa, Zea mays
Predicted reactivity Hordeum vulgare, Stipa sp. Triticum aestivum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-PIP1;3 antibodies

1.5 µg of crude membrane fraction/lane from Oryza sativa L. Akitakomachi  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIPs1;3 antibodies (AS09 504, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Below: Immunospecificity of anti.PIP1;3 antibodies to recombinant PIP proteins expressed in yeast cells. 1.5 µg protein/lane.

Most aquaporins show the dimerc forms in addition to monomer even in the presence of SDS.

 

 

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Related products

Related products

collection of antibodies to plasma membrane proteins

AS09 505 | anti-PIP1s | aquaporin

AS09 506 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1

AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1

AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5

Background

Background

PIP1;3 is a plasma membrane aquaporine which facilitates trasport of water across cell membrane. Alternative names: aquaporin PIP 1-3, water channel protein RWC3, RWC-3

Product citations

Selected references Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

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