PIP2;7 | Plasma membrane aquaporin, N-terminal

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2;7,P93004, At4g35100

29.7 | 30.5 kDa (Arabidopsis thaliana)

Application example

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;7 antibodies (AS09 469, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of plasma membranes are used, one µg load per well should be sufficient.

This antibody has a potential to work in immunolocalization studies, as it is recognizing N-terminall part of the sequence, which is exposed to the cytoplasm.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Theoretically this antibody can also react with PIP2;8 but it has not been confirmed experimentally.

AS09 488 | Anti-PIP2;1 | aquaporin PIP2;1, rabbit antibodies

AS09 490 | Anti-PIP2;2 | plasma membrane aquaporin 2b, rabbit antibodies

AS09 491 | Anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodies

Plant protein extraction buffer

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a