PIP2;7 | Plasma membrane aquaporin, N-terminal

AS09 469 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Jatropha curcas

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2;7,P93004, At4g35100

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)

Expected | apparent MW

29.7 | 30.5 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Jatropha curcas L. cv. "Biji Jarak "

Predicted reactivity Brassica oleracea, Juglans regia, Populus tremulax Populus tremuloides, Spinacia oleracea, Vitis vinifera

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

western blot using anti-PIP2;7 antibodies

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;7 antibodies (AS09 469, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of plasma membranes are used, one µg load per well should be sufficient.

This antibody has a potential to work in immunolocalization studies, as it is recognizing N-terminall part of the sequence, which is exposed to the cytoplasm.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Theoretically this antibody can also react with PIP2;8 but it has not been confirmed experimentally.

Related products

AS09 488 | Anti-PIP2;1 | aquaporin PIP2;1, rabbit antibodies

AS09 490 | Anti-PIP2;2 | plasma membrane aquaporin 2b, rabbit antibodies

AS09 491 | Anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodies

Plant protein extraction buffer

Background

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a

Product citations

Selected references Pou et al. (2016). Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7. Plant Mol Biol. 2016 Sep 26.
Hachez et al. (2014). The Arabidopsis Abiotic Stress-Induced TSPO-Related Protein Reduces Cell-Surface Expression of the Aquaporin PIP2;7 through Protein-Protein Interactions and Autophagic Degradation. Plant Cell. 2014 Dec;26(12):4974-90. doi: 10.1105/tpc.114.134080. Epub 2014 Dec 23.
Jang et al. (2013). Twoaquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.