PIP2;3 | Aquaporin, plasma membrane intrinistic protein 2-3

AS09 506 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa, Zea mays

PIP2;3 | Aquaporin, plasma membrane intrinistic protein 2-3 in the group Antibodies Plant/Algal  / Membrane Transport System / Plasma membrane at Agrisera AB (Antibodies for research) (AS09 506)
PIP2;3 | Aquaporin, plasma membrane intrinistic protein 2-3


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Product Information


KLH-conjugated peptide derived from Oryza sativa PIP2;3, UniProt: Q7XUA6

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Chromogenic Immunohistochemistry (CIH), ELISA (ELISA), Immunogold (IG), Western blot (WB)
Recommended dilution 1: 50 (CIH), 1 : 8000 (ELISA), 1 : 100 (IG), 1 : 2000 (WB)
Expected | apparent MW 31,9 | 26-29 kDa


Confirmed reactivity Oryza sativa, Zea mays
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in Arabidopsis thaliana, Hordeum vulgare, Solanum tuberosum

Application examples

Application examples

Application example

western blot detection using anti-PIP2;1

0.5 µg of crude membrane fraction/lane from Oryza sativa  L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;1 antibodies (AS09 506, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. This antibody will recognize PIP2;3 in root tissue only. No signal is obtained for leaf tissue. 

For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.

Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Related products

Related products

collection of antibodies to plasma membrane proteins

AS09 505 | Anti-PIP1s | aquaporin, rabbit antibodies

AS09 507 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodies

AS09 508 | Anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5, rabbit antibodies

Plant protein extraction buffer



PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane.

Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3

Product citations

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