Plant Vitamin B12 ELISA quantitation kit
AS15 3025 | Reactivity: Plant Vitamin B12
Background | This ELISA immunoassay utilize the principle of competitive inhibition for quantitative determination of endogenous Vitamin B12 concentrations in plant tissues.
An antibody specific to Vitamin B12 is pre-coated on the surface of the micro titer plate. Standards or samples are added to the plate wells together with Vitamin B12, conjugated to biotin. The reaction of competitive inhibition is initiated between the Vitamin B12 in the samples and the biotin-conjugated Vitamin B12 with the pre-coated Vitamin B12 antibody. After washing the plate, avidin-conjugated HRP is added followed by another wash. A substrate solution is then added and the color develops which is inversely proportional to the amount of Vitamin B12 in the sample. The intensity of the color is measured after the reaction is stopped.
This Plant Vitamin B12 ELISA kit contains the following reagents:
Assay plate: 1x (96 wells)
Standards: 2 x 0.5 ml
Sample diluent: 2 x 20 ml
Biotin-conjugate: 1 x 600 µl (10 x concentrate)
Biotin conjugate diluent: 1 x 10 ml
HRP-avidin: 1 x 120 ml
HRP-avidin Diluent: 1 x 20 ml
Washing Buffer: 1 x 20 ml (25 x concentrate)
TMB Substrate: 1 x 10 ml (store in the dark)
Stop Solution: 1 x 10 ml (contains acid, wear eye, hand, face and clothing protection)
Adhesive Strip: 4x (for 96 wells)
This kit is for research purpose only.
Storage: Store at 2-8°C. Opened kit can be stored at 2-8°C for maximum of 1 month.
Expiry date: Kit should not be used beyond expiry date.
Detection range: 15.6-1000 pg/ml
Specificity/Sensitivity: High specificity and sensitivity for plant Vitamin A. No significant cross reactivity has been observed.
AS15 3024 | Plant Vitamin A ELISA quantitation kit