Name:
Phone:
E-mail:
Message

IAA | indole-3-acetic acid (N1) (for immunolocalization)

AS09 421 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Eucalyptus globulus, Pinus radiata, Vitis vinifera

IAA | indole-3-acetic acid (N1) (for immunolocalization) in the group Plant/Algal Antibodies / Hormones / Auxins at Agrisera AB (Antibodies for research) (AS09 421)

PRODUCT INFORMATION IN PDF

Qty: 
278 €

Info: Add review
product information
Background

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in  in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.

Immunogen

BSA-conjugated, via N1 group of indole, indole-3-acetic acid (N1)

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified in PBS pH 7.4
Format Liquid
Quantity 200 µg
Reconstitution
Storage

Freeze upol arrival and store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunolocalization (IL)
Related products

AS06 193| anti- IAA

AS09 420 | anti- indole-3-acetic acid (C1') for ELISA

Secondary antibodies

Additional information

Useful references for immunolocalization work:
DeWitte et al. (1999). Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation. Plant Physiol. 119:111-121.
Shiozaki et al. (1999). Localisation of abscisic acid in grape berry by immunohitochemical techniques. J.Japan Hort.Sci.68:1-9. 
Skene et al. (1987). Model systems for the immunolocalisation of cis, trans-abscisic acid in plant tissues. Planta 172:192-199.
Sosountozov et al. (1986). Immunoelectron-microscopy localisation of abscisic acid with collodial gold on Lowicryl-embeded tissues of Chenopodium polyspermum L. Planta 168: 471-481.
Zavala et al. (1983). Localisation of a phytohormone using immunocytochemistry. J.Cell Biol. 97:1235-1239.

application information
Recommended dilution Specific information about dilution is going to be included on the vial.
Expected | apparent MW
Confirmed reactivity indole-3-acetic acid (N1) in Eucalyptus globulus, Pinus radiata, Vitis vinifera
Predicted reactivity Indole-3-acetic acid (N1)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Antibody can be used for direct detection of free IAA (see reference below)

Antibody is provided in 50% glycerol. For larger quantity (1 mg) or for  ELISA kit - please inquire.

Selected references Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007.
Escandón et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.
Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56–66.
De Diego et al. (2013).Immunolocalization ofIAA andABA inroots andneedles ofradiatapine (Pinusradiata) duringdrought andrewatering. Tree Physiol., May; 33(5):537-549.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com